Refereed journal article or data article (A1)
PP2A-B'γ modulates foliar trans-methylation capacity and the formation of 4-methoxy-indol-3-yl-methyl glucosinolate in Arabidopsis leaves
List of Authors: Rahikainen Moona, Trotta Andrea, Alegre Garcia Sara, Pascual Jesus, Vuorinen Katariina, Overmyer Kirk, Moffatt Barbara, Ravanel Stephane, Glawischnig, Kangasjärvi Saijaliisa
Publisher: Wiley-Blackwell Publishing Ltd.
Publication year: 2017
Journal: Plant Journal
Volume number: 89
Issue number: 1
Start page: 112
End page: 127
Number of pages: 16
ISSN: 0960-7412
eISSN: 1365-313X
DOI: http://dx.doi.org/10.1111/tpj.13326
URL: http://onlinelibrary.wiley.com/doi/10.1111/tpj.13326/full
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/17743622
Glucosinolates (GSL) of cruciferous plants comprise a major group of
structurally diverse secondary compounds which act as deterrents against
aphids and microbial pathogens and have large commercial and ecological
impacts. While the transcriptional regulation governing the
biosynthesis and modification of GSL is now relatively well understood,
post-translational regulatory components that specifically determine the
structural variation of indole glucosinolates have not been reported.
We show that the cytoplasmic protein phosphatase 2A regulatory subunit
B'γ (PP2A-B'γ) physically interacts with indole glucosinolate
methyltransferases and controls the methoxylation of indole
glucosinolates and the formation of 4-methoxy-indol-3-yl-methyl
glucosinolate in Arabidopsis leaves. By taking advantage of proteomic
approaches and metabolic analysis we further demonstrate that PP2A-B'γ
is required to control the abundance of oligomeric protein complexes
functionally linked with the activated methyl cycle and the
trans-methylation capacity of leaf cells. These findings highlight the
key regulatory role of PP2A-B'γ in methionine metabolism and provide a
previously unrecognized perspective for metabolic engineering of
glucosinolate metabolism in cruciferous plants.
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