Refereed journal article or data article (A1)

PP2A-B'γ modulates foliar trans-methylation capacity and the formation of 4-methoxy-indol-3-yl-methyl glucosinolate in Arabidopsis leaves




List of AuthorsRahikainen Moona, Trotta Andrea, Alegre Garcia Sara, Pascual Jesus, Vuorinen Katariina, Overmyer Kirk, Moffatt Barbara, Ravanel Stephane, Glawischnig, Kangasjärvi Saijaliisa

PublisherWiley-Blackwell Publishing Ltd.

Publication year2017

JournalPlant Journal

Volume number89

Issue number1

Start page112

End page127

Number of pages16

ISSN0960-7412

eISSN1365-313X

DOIhttp://dx.doi.org/10.1111/tpj.13326

URLhttp://onlinelibrary.wiley.com/doi/10.1111/tpj.13326/full

Self-archived copy’s web addresshttps://research.utu.fi/converis/portal/detail/Publication/17743622


Abstract

Glucosinolates (GSL) of cruciferous plants comprise a major group of
structurally diverse secondary compounds which act as deterrents against
aphids and microbial pathogens and have large commercial and ecological
impacts. While the transcriptional regulation governing the
biosynthesis and modification of GSL is now relatively well understood,
post-translational regulatory components that specifically determine the
structural variation of indole glucosinolates have not been reported.
We show that the cytoplasmic protein phosphatase 2A regulatory subunit
B'γ (PP2A-B'γ) physically interacts with indole glucosinolate
methyltransferases and controls the methoxylation of indole
glucosinolates and the formation of 4-methoxy-indol-3-yl-methyl
glucosinolate in Arabidopsis leaves. By taking advantage of proteomic
approaches and metabolic analysis we further demonstrate that PP2A-B'γ
is required to control the abundance of oligomeric protein complexes
functionally linked with the activated methyl cycle and the
trans-methylation capacity of leaf cells. These findings highlight the
key regulatory role of PP2A-B'γ in methionine metabolism and provide a
previously unrecognized perspective for metabolic engineering of
glucosinolate metabolism in cruciferous plants.


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Last updated on 2022-07-04 at 16:22