A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
COX7A2L genetic variants determine cardiorespiratory fitness in mice and human
Tekijät: Benegiamo Giorgia, Bou Sleiman Maroun, Wohlwend Martin, Rodríguez-López Sandra, Goeminne Ludger J. E., Laurila Pirkka-Pekka, Klevjer Marie, Salonen Minna K., Lahti Jari, Jha Pooja, Cogliati Sara, Enriquez José Antonio, Brumpton Ben M., Bye Anja, Eriksson Johan G., Auverx Johan
Kustantaja: Nature Research
Julkaisuvuosi: 2022
Journal: Nature Metabolism
Tietokannassa oleva lehden nimi: NATURE METABOLISM
Lehden akronyymi: Nat Metab
Vuosikerta: 4
Aloitussivu: 1336
Lopetussivu: 1351
Sivujen määrä: 27
eISSN: 2522-5812
DOI: https://doi.org/10.1038/s42255-022-00655-0
Verkko-osoite: https://www.nature.com/articles/s42255-022-00655-0
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/177030991
Benegiamo et al. identify genetic variants of the mitochondrial supercomplex assembly factor COX7A2L in the skeletal muscle of mice and humans that promote cardiorespiratory fitness.Mitochondrial respiratory complexes form superassembled structures called supercomplexes. COX7A2L is a supercomplex-specific assembly factor in mammals, although its implication for supercomplex formation and cellular metabolism remains controversial. Here we identify a role for COX7A2L for mitochondrial supercomplex formation in humans. By using human cis-expression quantitative trait loci data, we highlight genetic variants in the COX7A2L gene that affect its skeletal muscle expression specifically. The most significant cis-expression quantitative trait locus is a 10-bp insertion in the COX7A2L 3 ' untranslated region that increases messenger RNA stability and expression. Human myotubes harboring this insertion have more supercomplexes and increased respiration. Notably, increased COX7A2L expression in the muscle is associated with lower body fat and improved cardiorespiratory fitness in humans. Accordingly, specific reconstitution of Cox7a2l expression in C57BL/6J mice leads to higher maximal oxygen consumption, increased lean mass and increased energy expenditure. Furthermore, Cox7a2l expression in mice is induced specifically in the muscle upon exercise. These findings elucidate the genetic basis of mitochondrial supercomplex formation and function in humans and show that COX7A2L plays an important role in cardiorespiratory fitness, which could have broad therapeutic implications in reducing cardiovascular mortality.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
