A1 Refereed original research article in a scientific journal
Upconversion FRET quantitation: the role of donor photoexcitation mode and compositional architecture on the decay and intensity based responses
Authors: Kotulska Agata M., Pilch-Wróbel Aleksandra, Lahtinen Satu, Soukka Tero, Bednarkiewicz Artur
Publisher: SPRINGERNATURE
Publication year: 2022
Journal: Light: Science and Applications
Journal name in source: LIGHT-SCIENCE & APPLICATIONS
Journal acronym: LIGHT-SCI APPL
Article number: 256
Volume: 11
Number of pages: 14
ISSN: 2095-5545
eISSN: 2047-7538
DOI: https://doi.org/10.1038/s41377-022-00946-x
Web address : https://www.nature.com/articles/s41377-022-00946-x
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/176276720
Lanthanide-doped colloidal nanoparticles capable of photon upconversion (UC) offer long luminescence lifetimes, narrowband absorption and emission spectra, and efficient anti-Stokes emission. These features are highly advantageous for Forster Resonance Energy Transfer (FRET) based detection. Upconverting nanoparticles (UCNPs) as donors may solve the existing problems of molecular FRET systems, such as photobleaching and limitations in quantitative analysis, but these new labels also bring new challenges. Here we have studied the impact of the core-shell compositional architecture of upconverting nanoparticle donors and the mode of photoexcitation on the performance of UC-FRET from UCNPs to Rose Bengal (RB) molecular acceptor. We have quantitatively compared luminescence rise and decay kinetics of Er3+ emission using core-only NaYF4: 20% Yb, 2% Er and core-shell NaYF4: 20% Yb @ NaYF4: 20% Yb, 5% Er donor UCNPs under three photoexcitation schemes: (1) direct short-pulse photoexcitation of Er3+ at 520 nm; indirect photoexcitation of Er3+ through Yb3+ sensitizer with (2) 980 nm short (5-7 ns) or (3) 980 nm long (4 ms) laser pulses. The donor luminescence kinetics and steady-state emission spectra differed between the UCNP architectures and excitation schemes. Aiming for highly sensitive kinetic upconversion FRET-based biomolecular assays, the experimental results underline the complexity of the excitation and energy-migration mechanisms affecting the Er3+ donor responses and suggest ways to optimize the photoexcitation scheme and the architecture of the UCNPs used as luminescent donors.
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