A1 Refereed original research article in a scientific journal
Techno-functional properties of protein from protease-treated brewers’ spent grain (BSG) and investigation of antioxidant activity of extracted proteins and BSG residues
Authors: Naibaho Joncer, Korzeniowska Małgorzata, Wojdyło Aneta, Muchdatul Ayunda Hanif, Foste Maike, Yang Baoru
Publisher: Academic Press
Publication year: 2022
Journal: Journal of Cereal Science
Journal name in source: Journal of Cereal Science
Article number: 103524
Volume: 107
eISSN: 1095-9963
DOI: https://doi.org/10.1016/j.jcs.2022.103524
Web address : https://doi.org/10.1016/j.jcs.2022.103524
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/176037702
The study aimed to investigate the biological properties of the protein fraction of brewers’ spent grain (BSG) and its sediments as well as the techno-functional properties of BSG protein (BSGP). BSG was incubated with 0.5% protamex and a combination of protamex and flavourzyme, in addition to the control (incubation without protease). The results showed that enzymatic treatment enhanced the antioxidant activity of BSGP. Compared to the sediment fraction, BSGPs had higher antioxidant capacities than those in sediments. The current study demonstrated that the FRAP value is aligned with the amount of the polyphenolic compounds, while BSGP is responsible for ORAC and ABTS capabilities. Enzyme treatment on BSG enhanced the antioxidant properties of BSGPs and the amount of the phenolic compounds of the sediments. BSGPs treated with proteases possessed higher oil-holding capacity, foaming properties and lower emulsion capability. In conclusion, enzymatic treatment of BSG enhanced the protein functionality and bioactivity as well as intensified the antioxidant activity of its sediments allowing further valorization.
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