New faunistic and taxonomic data are provided for 15 families of Iranian spiders. Two lycosid species, Alopecosa murphyorum sp. n. (♂, Semnan Province) and Alopecosa robertsi sp. n. (♂, Zanjan Province), are described as new to science. Nine species are recorded from Iran for the first time, and 182 new provincial records are provided for 53 species. Among new distributional data, the first confirmed record of the family Leptonetidae in Iran, representing its easternmost record in the West Palaearctic, is provided, as well as a redescription of the recently described Evippa amitaii Armiach Steinpress, Alderweireldt, Cohen, Chipman & Gavish-Regev, 2021 (Lycosidae), and illustrations for the examined type material of Evippa luteipalpis Roewer, 1955 (Lycosidae). Two species, Aulonia albimana (Walckenaer, 1805) (Lycosidae) and Tetragnatha javana (Thorell, 1890) (Tetragnathidae) are removed from the list of Iranian spiders, while Piratula insularis (Emerton, 1885) (Lycosidae) is removed from the list of Turkish spiders. Furthermore, Steatoda paykulliana (Walckenaer, 1806) (Theridiidae) is recorded from India for the first time. The newly described and some other species representing new country or provincial records are illustrated.