A1 Refereed original research article in a scientific journal
Rapid high-throughput compatible label-free virus particle quantification method based on time-resolved luminescence
Authors: Kopra Kari, Hassan Nazia, Vuorinen Emmiliisa, Valtonen Salla, Mahran Randa, Habib Huda, Jalkanen Pinja, Susi Petri, Hytönen Vesa, Hankaniemi Minna, Ylä-Herttuala Seppo, Kakkola Laura, Peurla Markus, Härmä Harri
Publisher: SPRINGER HEIDELBERG
Publication year: 2022
Journal: Analytical and Bioanalytical Chemistry
Journal name in source: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Journal acronym: ANAL BIOANAL CHEM
Volume: 414
Issue: 15
First page : 4509
Last page: 4518
Number of pages: 10
ISSN: 1618-2642
eISSN: 1618-2650
DOI: https://doi.org/10.1007/s00216-022-04104-5
Web address : https://link.springer.com/article/10.1007/s00216-022-04104-5
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/175390176
Viruses play a major role in modern society and create risks from global pandemics and bioterrorism to challenges in agriculture. Virus infectivity assays and genome copy number determination methods are often used to obtain information on virus preparations used in diagnostics and vaccine development. However, these methods do not provide information on virus particle count. Current methods to measure the number of viral particles are often cumbersome and require highly purified virus preparations and expensive instrumentation. To tackle these problems, we developed a simple and cost-effective time-resolved luminescence-based method for virus particle quantification. This mix-and-measure technique is based on the recognition of the virus particles by an external Eu3+-peptide probe, providing results on virus count in minutes. The method enables the detection of non-enveloped and enveloped viruses, having over tenfold higher detectability for enveloped, dynamic range from 5E6 to 3E10 vp/mL, than non-enveloped viruses. Multiple non-enveloped and enveloped viruses were used to demonstrate the functionality and robustness of the Protein-Probe method.
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