Mussel-Inspired and Bioclickable Peptide Engineered Surface to Combat Thrombosis and Infection
: Mou Xiaohui, Zhang Hongbo, Qiu Hua, Zhang Wentai, Wang Ying, Xiong Kaiqin, Huang Nan, Santos Hélder A., Yang Zhilu
Publisher: AMER ASSOC ADVANCEMENT SCIENCE
: 2022
: Research
: RESEARCH
: RESEARCH-CHINA
: 9780879
: 2022
: 14
: 2096-5168
DOI: https://doi.org/10.34133/2022/9780879
: https://spj.sciencemag.org/journals/research/2022/9780879/
: https://research.utu.fi/converis/portal/detail/Publication/175238675
Thrombosis and infections are the two major complications associated with extracorporeal circuits and indwelling medical devices, leading to significant mortality in clinic. To address this issue, here, we report a biomimetic surface engineering strategy by the integration of mussel-inspired adhesive peptide, with bio-orthogonal click chemistry, to tailor the surface functionalities of tubing and catheters. Inspired by mussel adhesive foot protein, a bioclickable peptide mimic (DOPA)(4)-azide-based structure is designed and grafted on an aminated tubing robustly based on catechol-amine chemistry. Then, the dibenzylcyclooctyne (DBCO) modified nitric oxide generating species of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) chelated copper ions and the DBCO-modified antimicrobial peptide (DBCO-AMP) are clicked onto the grafted surfaces via bio-orthogonal reaction. The combination of the robustly grafted AMP and Cu-DOTA endows the modified tubing with durable antimicrobial properties and ability in long-term catalytically generating NO from endogenous s-nitrosothiols to resist adhesion/activation of platelets, thus preventing the formation of thrombosis. Overall, this biomimetic surface engineering technology provides a promising solution for multicomponent surface functionalization and the surface bioengineering of biomedical devices with enhanced clinical performance.
UTU Research Portal