A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
The DNA polymerase of bacteriophage YerA41 replicates its T-modified DNA in a primer-independent manner
Tekijät: Gomez-Raya-Vilanova Miguel V, Leskinen Katarzyna, Bhattacharjee Arnab, Virta Pasi, Rosenqvist Petja, Smith Jake LR, Bayfield Oliver W, Homberger Christina, Kerrinnes Tobias, Vogel Jörg, Pajunen Maria I, Skurnik Mikael
Kustantaja: OXFORD UNIV PRESS
Julkaisuvuosi: 2022
Journal: Nucleic Acids Research
Tietokannassa oleva lehden nimi: NUCLEIC ACIDS RESEARCH
Lehden akronyymi: NUCLEIC ACIDS RES
Vuosikerta: 50
Numero: 7
Aloitussivu: 3985
Lopetussivu: 3997
Sivujen määrä: 13
ISSN: 0305-1048
DOI: https://doi.org/10.1093/nar/gkac203
Verkko-osoite: https://academic.oup.com/nar/article/50/7/3985/6561659
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/175191148
Yersinia phage YerA41 is morphologically similar to jumbo bacteriophages. The isolated genomic material of YerA41 could not be digested by restriction enzymes, and used as a template by conventional DNA polymerases. Nucleoside analysis of the YerA41 genomic material, carried out to find out whether this was due to modified nucleotides, revealed the presence of a ca 1 kDa substitution of thymidine with apparent oligosaccharide character. We identified and purified the phage DNA polymerase (DNAP) that could replicate the YerA41 genomic DNA even without added primers. Cryo-electron microscopy (EM) was used to characterize structural details of the phage particle. The storage capacity of the 131 nm diameter head was calculated to accommodate a significantly longer genome than that of the 145 577 bp genomic DNA of YerA41 determined here. Indeed, cryo-EM revealed, in contrast to the 25 angstrom in other phages, spacings of 33-36 angstrom between shells of the genomic material inside YerA41 heads suggesting that the heavily substituted thymidine increases significantly the spacing of the DNA packaged inside the capsid. In conclusion, YerA41 appears to be an unconventional phage that packages thymidine-modified genomic DNA into its capsids along with its own DNAP that has the ability to replicate the genome.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
