Refereed journal article or data article (A1)

Potential pathobionts in vaginal microbiota are affected by fish oil and/or probiotics intervention in overweight and obese pregnant women




List of Authors: Houttu Noora, Mokkala Kati, Tareeq Saleem Wisam, Virtanen Seppo, Juhila Juuso, Koivuniemi Ella, Pellonperä Outi, Tertti Kristiina, Luokola Paula, Sorsa Timo, Salonen Anne, Lahti Leo, Laitinen Kirsi

Publisher: Elsevier Masson s.r.l.

Publication year: 2022

Journal: Biomedicine and Pharmacotherapy

Journal name in source: Biomedicine and Pharmacotherapy

Volume number: 149

ISSN: 0753-3322

eISSN: 1950-6007

DOI: http://dx.doi.org/10.1016/j.biopha.2022.112841

URL: https://doi.org/10.1016/j.biopha.2022.112841

Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/175136884


Abstract

New means to stabilize the microbial balance during pregnancy could benefit maternal health. Our objectives were to investigate in overweight/obese pregnant women 1) the impact of long-chain polyunsaturated fatty acids (fish oil) and/or probiotics on the vaginal microbiota, 2) its relation to gestational diabetes mellitus (GDM) and 3) its interaction with vaginal active matrix metalloproteinase-8 (aMMP-8) and serum high sensitivity C-reactive protein (hsCRP) and phosphorylated insulin-like growth factor-binding protein-1 (phIGFBP-1), IGFBP-1 and aMMP-8.

The women were allocated to fish oil + placebo, probiotics + placebo, fish oil + probiotics and placebo + placebo-groups, from early pregnancy onwards (fish oil: 1.9 g docosahexaenoic acid and 0.22 g eicosapentaenoic acid; probiotics: Lacticaseibacillus rhamnosus HN001 (formerly Lactobacillus rhamnosus HN001) and Bifidobacterium animalis ssp. lactis 420, 1010 colony-forming units each). Vaginal and serum samples (early pregnancy, n = 112; late pregnancy, n = 116), were analyzed for vaginal microbiota using 16S rRNA gene amplicon sequencing and vaginal aMMP-8 and serum hsCRP, aMMP-8, phIGFBP-1 and IGFBP-1 by immunoassays. GDM was diagnosed from a 2-h 75 g OGTT. ClinicalTrials.gov, NCT01922791.

The intervention exerted effects on many low-abundant bacteria. Compared to the placebo-group, there was a lower abundance of potential pathobionts, namely Ureaplasma urealyticum in the fish oil-group, Ureaplasma, U. urealyticum and Prevotella disiens in the probiotics-group, Dialister invisus and Prevotella timonensis in the fish oil + probiotics-group. Moreover, probiotics decreased the abundance of a few potential pathobionts during pregnancy. Many bacteria were related to GDM. The vaginal aMMP-8 level correlated significantly with α-diversity and inversely with two Lactobacillus species.

Dietary interventions, especially probiotics, may have beneficial effects on the vaginal microbiota during pregnancy.


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Last updated on 2022-11-05 at 14:43