A1 Refereed original research article in a scientific journal

Potential pathobionts in vaginal microbiota are affected by fish oil and/or probiotics intervention in overweight and obese pregnant women




AuthorsHouttu Noora, Mokkala Kati, Tareeq Saleem Wisam, Virtanen Seppo, Juhila Juuso, Koivuniemi Ella, Pellonperä Outi, Tertti Kristiina, Luokola Paula, Sorsa Timo, Salonen Anne, Lahti Leo, Laitinen Kirsi

PublisherElsevier Masson s.r.l.

Publication year2022

JournalBiomedicine and Pharmacotherapy

Journal name in sourceBiomedicine and Pharmacotherapy

Article number112841

Volume149

ISSN0753-3322

eISSN1950-6007

DOIhttps://doi.org/10.1016/j.biopha.2022.112841(external)

Web address https://doi.org/10.1016/j.biopha.2022.112841(external)

Self-archived copy’s web addresshttps://research.utu.fi/converis/portal/detail/Publication/175136884(external)


Abstract

New means to stabilize the microbial balance during pregnancy could benefit maternal health. Our objectives were to investigate in overweight/obese pregnant women 1) the impact of long-chain polyunsaturated fatty acids(external) (fish oil) and/or probiotics(external) on the vaginal microbiota(external), 2) its relation to gestational diabetes mellitus(external) (GDM) and 3) its interaction with vaginal active matrix metalloproteinase-8 (aMMP-8) and serum high sensitivity C-reactive protein (hsCRP) and phosphorylated insulin-like growth factor-binding protein-1 (phIGFBP-1), IGFBP-1 and aMMP-8.

The women were allocated to fish oil + placebo, probiotics + placebo, fish oil + probiotics and placebo + placebo-groups, from early pregnancy(external) onwards (fish oil: 1.9 g docosahexaenoic acid(external) and 0.22 g eicosapentaenoic acid(external); probiotics: Lacticaseibacillus rhamnosus HN001 (formerly Lactobacillus(external) rhamnosus HN001) and Bifidobacterium animalis(external) ssp. lactis 420, 1010 colony-forming units each). Vaginal and serum samples (early pregnancy, n = 112; late pregnancy(external), n = 116), were analyzed for vaginal microbiota using 16S rRNA(external) gene amplicon(external) sequencing and vaginal aMMP-8 and serum hsCRP, aMMP-8, phIGFBP-1 and IGFBP-1 by immunoassays(external). GDM was diagnosed from a 2-h 75 g OGTT(external). ClinicalTrials.gov, NCT01922791.

The intervention exerted effects on many low-abundant bacteria. Compared to the placebo-group, there was a lower abundance of potential pathobionts, namely Ureaplasma(external) urealyticum in the fish oil-group, Ureaplasma, U. urealyticum and Prevotella(external) disiens in the probiotics-group, Dialister(external) invisus and Prevotella timonensis in the fish oil + probiotics-group. Moreover, probiotics decreased the abundance of a few potential pathobionts during pregnancy. Many bacteria were related to GDM. The vaginal aMMP-8 level correlated significantly with α-diversity and inversely with two Lactobacillus species.

Dietary interventions, especially probiotics, may have beneficial effects on the vaginal microbiota during pregnancy.


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Last updated on 2024-26-11 at 18:03