A1 Refereed original research article in a scientific journal
Potential pathobionts in vaginal microbiota are affected by fish oil and/or probiotics intervention in overweight and obese pregnant women
Authors: Houttu Noora, Mokkala Kati, Tareeq Saleem Wisam, Virtanen Seppo, Juhila Juuso, Koivuniemi Ella, Pellonperä Outi, Tertti Kristiina, Luokola Paula, Sorsa Timo, Salonen Anne, Lahti Leo, Laitinen Kirsi
Publisher: Elsevier Masson s.r.l.
Publication year: 2022
Journal: Biomedicine and Pharmacotherapy
Journal name in source: Biomedicine and Pharmacotherapy
Article number: 112841
Volume: 149
ISSN: 0753-3322
eISSN: 1950-6007
DOI: https://doi.org/10.1016/j.biopha.2022.112841(external)
Web address : https://doi.org/10.1016/j.biopha.2022.112841(external)
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/175136884(external)
New means to stabilize the microbial balance during pregnancy could benefit maternal health. Our objectives were to investigate in overweight/obese pregnant women 1) the impact of long-chain polyunsaturated fatty acids(external) (fish oil) and/or probiotics(external) on the vaginal microbiota(external), 2) its relation to gestational diabetes mellitus(external) (GDM) and 3) its interaction with vaginal active matrix metalloproteinase-8 (aMMP-8) and serum high sensitivity C-reactive protein (hsCRP) and phosphorylated insulin-like growth factor-binding protein-1 (phIGFBP-1), IGFBP-1 and aMMP-8.
The women were allocated to fish oil + placebo, probiotics + placebo, fish oil + probiotics and placebo + placebo-groups, from early pregnancy(external) onwards (fish oil: 1.9 g docosahexaenoic acid(external) and 0.22 g eicosapentaenoic acid(external); probiotics: Lacticaseibacillus rhamnosus HN001 (formerly Lactobacillus(external) rhamnosus HN001) and Bifidobacterium animalis(external) ssp. lactis 420, 1010 colony-forming units each). Vaginal and serum samples (early pregnancy, n = 112; late pregnancy(external), n = 116), were analyzed for vaginal microbiota using 16S rRNA(external) gene amplicon(external) sequencing and vaginal aMMP-8 and serum hsCRP, aMMP-8, phIGFBP-1 and IGFBP-1 by immunoassays(external). GDM was diagnosed from a 2-h 75 g OGTT(external). ClinicalTrials.gov, NCT01922791.
The intervention exerted effects on many low-abundant bacteria. Compared to the placebo-group, there was a lower abundance of potential pathobionts, namely Ureaplasma(external) urealyticum in the fish oil-group, Ureaplasma, U. urealyticum and Prevotella(external) disiens in the probiotics-group, Dialister(external) invisus and Prevotella timonensis in the fish oil + probiotics-group. Moreover, probiotics decreased the abundance of a few potential pathobionts during pregnancy. Many bacteria were related to GDM. The vaginal aMMP-8 level correlated significantly with α-diversity and inversely with two Lactobacillus species.
Dietary interventions, especially probiotics, may have beneficial effects on the vaginal microbiota during pregnancy.
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