A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä

Quantifying RNA synthesis at rate-limiting steps of transcription using nascent RNA-sequencing data




TekijätRabenius Adelina, Chandrakumaran Sajitha, Sistonen Lea, Vihervaara Anniina

KustantajaCell Press

Julkaisuvuosi2022

JournalSTAR protocols

Tietokannassa oleva lehden nimiSTAR Protocols

Artikkelin numero101036

Vuosikerta3

Numero1

eISSN2666-1667

DOIhttps://doi.org/10.1016/j.xpro.2021.101036

Verkko-osoitehttps://doi.org/10.1016/j.xpro.2021.101036

Rinnakkaistallenteen osoitehttps://research.utu.fi/converis/portal/detail/Publication/175006010


Tiivistelmä

Nascent RNA-sequencing tracks transcription at nucleotide resolution. The genomic distribution of engaged transcription complexes, in turn, uncovers functional genomic regions. Here, we provide analytical steps to (1) identify transcribed regulatory elements de novo genome-wide, (2) quantify engaged transcription complexes at enhancers, promoter-proximal regions, divergent transcripts, gene bodies, and termination windows, and (3) measure distribution of transcription machineries and regulatory proteins across functional genomic regions. This protocol tracks engaged transcription complexes across functional genomic regions demonstrated in human K562 erythroleukemia cells.


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Last updated on 2025-27-03 at 21:55