A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Quantifying RNA synthesis at rate-limiting steps of transcription using nascent RNA-sequencing data
Tekijät: Rabenius Adelina, Chandrakumaran Sajitha, Sistonen Lea, Vihervaara Anniina
Kustantaja: Cell Press
Julkaisuvuosi: 2022
Journal: STAR protocols
Tietokannassa oleva lehden nimi: STAR Protocols
Artikkelin numero: 101036
Vuosikerta: 3
Numero: 1
eISSN: 2666-1667
DOI: https://doi.org/10.1016/j.xpro.2021.101036
Verkko-osoite: https://doi.org/10.1016/j.xpro.2021.101036
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/175006010
Nascent RNA-sequencing tracks transcription at nucleotide resolution. The genomic distribution of engaged transcription complexes, in turn, uncovers functional genomic regions. Here, we provide analytical steps to (1) identify transcribed regulatory elements de novo genome-wide, (2) quantify engaged transcription complexes at enhancers, promoter-proximal regions, divergent transcripts, gene bodies, and termination windows, and (3) measure distribution of transcription machineries and regulatory proteins across functional genomic regions. This protocol tracks engaged transcription complexes across functional genomic regions demonstrated in human K562 erythroleukemia cells.
Ladattava julkaisu This is an electronic reprint of the original article. |