A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Detecting disease associated biomarkers by luminescence modulating phages
Tekijät: Kulpakko Janne, Juusti Vilhelmiina, Rannikko Antti, Hänninen Pekka E
Kustantaja: NATURE PORTFOLIO
Julkaisuvuosi: 2022
Journal: Scientific Reports
Tietokannassa oleva lehden nimi: SCIENTIFIC REPORTS
Lehden akronyymi: SCI REP-UK
Artikkelin numero: 2433
Vuosikerta: 12
Sivujen määrä: 8
ISSN: 2045-2322
eISSN: 2045-2322
DOI: https://doi.org/10.1038/s41598-022-06433-y
Verkko-osoite: https://www.nature.com/articles/s41598-022-06433-y
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/174961434
Assessment of risk for a given disease and the diagnosis of diseases is often based on assays detecting biomarkers. Antibody-based biomarker-assays for diseases such as prostate cancer are often ambiguous and biomarker proteins are frequently also elevated for reasons that are unspecific. We have opted to use luminescence modulating phages for the analysis of known acute inflammatory response biomarker CRP (C-reactive protein) and biomarkers of prostate cancer in urine samples. Firstly, CRP was used to simulate the detection process in a controlled chemical environment. Secondly, we tried to classify more challenging lethal prostate cancer samples from control samples. Our unique method utilizes a special biopanning process in order to create special phages capable of capturing a dye necessary for detection and potential biomarkers. As the biomarker-molecules interfere with the phages, dye is repelled from the phage network resulting in an altered reporter luminescence. These changes can be observed with an absorbance reader and even with the naked eye. The simple method could present an alternative for screening of disease biomarkers. For prostate cancer urine samples, we achieved a sensitivity of 80% and specificity of 75% to detect Grade Group (GG) 4 and 5 prostate cancer.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
