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Clamping of RNA with PNA enables targeting of microRNA.
Tekijät: Ghidini A, Bergquist H, Murtola M, Punga T , Zain R, Strömberg R
Kustantaja: ROYAL SOC CHEMISTRY
Julkaisuvuosi: 2016
Journal: Organic and Biomolecular Chemistry
Tietokannassa oleva lehden nimi: Organic & biomolecular chemistry
Lehden akronyymi: Org Biomol Chem
Vuosikerta: 14
Numero: 23
Aloitussivu: 5210
Lopetussivu: 5213
Sivujen määrä: -5206
ISSN: 1477-0539
eISSN: 1477-0539
DOI: https://doi.org/10.1039/c6ob00516k
Tiivistelmä
To be able to target microRNAs also at stages where these are in a double stranded or hairpin form we have studied BisPNA designed to clamp the target and give sufficient affinity to allow for strand invasion. We show that BisPNA complexes are more stable with RNA than with DNA. In addition, 24-mer BisPNA (AntimiR) constructs form complexes with a hairpin RNA that is a model of the microRNA miR-376b, suggesting that PNA-clamping may be an effective way of targeting microRNAs.
To be able to target microRNAs also at stages where these are in a double stranded or hairpin form we have studied BisPNA designed to clamp the target and give sufficient affinity to allow for strand invasion. We show that BisPNA complexes are more stable with RNA than with DNA. In addition, 24-mer BisPNA (AntimiR) constructs form complexes with a hairpin RNA that is a model of the microRNA miR-376b, suggesting that PNA-clamping may be an effective way of targeting microRNAs.
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