A1 Refereed original research article in a scientific journal
Clamping of RNA with PNA enables targeting of microRNA.
Authors: Ghidini A, Bergquist H, Murtola M, Punga T , Zain R, Strömberg R
Publisher: ROYAL SOC CHEMISTRY
Publication year: 2016
Journal: Organic and Biomolecular Chemistry
Journal name in source: Organic & biomolecular chemistry
Journal acronym: Org Biomol Chem
Volume: 14
Issue: 23
First page : 5210
Last page: 5213
Number of pages: -5206
ISSN: 1477-0539
eISSN: 1477-0539
DOI: https://doi.org/10.1039/c6ob00516k
Abstract
To be able to target microRNAs also at stages where these are in a double stranded or hairpin form we have studied BisPNA designed to clamp the target and give sufficient affinity to allow for strand invasion. We show that BisPNA complexes are more stable with RNA than with DNA. In addition, 24-mer BisPNA (AntimiR) constructs form complexes with a hairpin RNA that is a model of the microRNA miR-376b, suggesting that PNA-clamping may be an effective way of targeting microRNAs.
To be able to target microRNAs also at stages where these are in a double stranded or hairpin form we have studied BisPNA designed to clamp the target and give sufficient affinity to allow for strand invasion. We show that BisPNA complexes are more stable with RNA than with DNA. In addition, 24-mer BisPNA (AntimiR) constructs form complexes with a hairpin RNA that is a model of the microRNA miR-376b, suggesting that PNA-clamping may be an effective way of targeting microRNAs.
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