PCR primers for 30 novel gene regions in the nuclear genomes of Lepidoptera

: Wahlberg N, Pena C, Ahola M, Wheat CW, Rota J

Publisher: PENSOFT PUBL

: 2016

: ZooKeys

: ZOOKEYS

: 596

: 129

: 141

: 13

: 1313-2989

DOI: https://doi.org/10.3897/zookeys.596.8399(external)

We report primer pairs for 30 new gene regions in the nuclear genomes of Lepidoptera that can be amplified using a standard PCR protocol. The new primers were tested across diverse Lepidoptera, including nonditrysians and a wide selection of ditrysians. These new gene regions give a total of 11,043 bp of DNA sequence data and they show similar variability to traditionally used nuclear gene regions in studies of Lepidoptera. We feel that a PCR-based approach still has its place in molecular systematic studies of Lepidoptera, particularly at the intrafamilial level, and our new set of primers now provides a route to generating phylogenomic datasets using traditional methods.