A1 Refereed original research article in a scientific journal
PCR primers for 30 novel gene regions in the nuclear genomes of Lepidoptera
Authors: Wahlberg N, Pena C, Ahola M, Wheat CW, Rota J
Publisher: PENSOFT PUBL
Publication year: 2016
Journal: ZooKeys
Journal name in source: ZOOKEYS
Journal acronym: ZOOKEYS
Issue: 596
First page : 129
Last page: 141
Number of pages: 13
ISSN: 1313-2989
DOI: https://doi.org/10.3897/zookeys.596.8399
Abstract
We report primer pairs for 30 new gene regions in the nuclear genomes of Lepidoptera that can be amplified using a standard PCR protocol. The new primers were tested across diverse Lepidoptera, including nonditrysians and a wide selection of ditrysians. These new gene regions give a total of 11,043 bp of DNA sequence data and they show similar variability to traditionally used nuclear gene regions in studies of Lepidoptera. We feel that a PCR-based approach still has its place in molecular systematic studies of Lepidoptera, particularly at the intrafamilial level, and our new set of primers now provides a route to generating phylogenomic datasets using traditional methods.
We report primer pairs for 30 new gene regions in the nuclear genomes of Lepidoptera that can be amplified using a standard PCR protocol. The new primers were tested across diverse Lepidoptera, including nonditrysians and a wide selection of ditrysians. These new gene regions give a total of 11,043 bp of DNA sequence data and they show similar variability to traditionally used nuclear gene regions in studies of Lepidoptera. We feel that a PCR-based approach still has its place in molecular systematic studies of Lepidoptera, particularly at the intrafamilial level, and our new set of primers now provides a route to generating phylogenomic datasets using traditional methods.
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