A1 Refereed original research article in a scientific journal
Structural basis of L-phosphoserine binding to Bacillus alcalophilus phosphoserine aminotransferase
Authors: Battula P, Dubnovitsky AP, Papageorgiou AC
Publisher: WILEY-BLACKWELL
Publication year: 2013
Journal: Acta Crystallographica Section D: Biological Crystallography
Journal name in source: ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
Journal acronym: ACTA CRYSTALLOGR D
Number in series: 5
Volume: 69
Issue: 5
First page : 804
Last page: 811
Number of pages: 8
ISSN: 0907-4449
DOI: https://doi.org/10.1107/S0907444913002096
Web address : http://journals.iucr.org/d/issues/2013/05/00/tz5018/tz5018sup1.pdf
Abstract
Phosphoserine aminotransferase is a vitamin B-6-dependent enzyme that catalyzes the reversible conversion of 3-phosphohydroxypyruvate to l-phosphoserine using glutamate as an amine donor. In an effort to gain insight into the substrate-recognition mechanism of the enzyme, crystal structures of Bacillus alcalophilus phosphoserine aminotransferase in the presence or absence of l-phosphoserine were determined to resolutions of 1.5 and 1.6 angstrom, respectively. Local conformational changes induced upon substrate binding were identified. However, in contrast to other aminotransferases, no domain or subunit movements were observed. Two Arg residues (Arg42 and Arg328) and two His residues (His41 and His327) were found to form a tight binding site for the phosphate group of l-phosphoserine. Comparison with Escherichia coli phosphoserine aminotransferase in complex with the substrate analogue alpha-methylglutamate revealed more extensive structural changes in the case of l-phosphoserine binding. Based on the structural analysis, the flexibility of Arg328 is proposed to be critical for substrate recognition.
Phosphoserine aminotransferase is a vitamin B-6-dependent enzyme that catalyzes the reversible conversion of 3-phosphohydroxypyruvate to l-phosphoserine using glutamate as an amine donor. In an effort to gain insight into the substrate-recognition mechanism of the enzyme, crystal structures of Bacillus alcalophilus phosphoserine aminotransferase in the presence or absence of l-phosphoserine were determined to resolutions of 1.5 and 1.6 angstrom, respectively. Local conformational changes induced upon substrate binding were identified. However, in contrast to other aminotransferases, no domain or subunit movements were observed. Two Arg residues (Arg42 and Arg328) and two His residues (His41 and His327) were found to form a tight binding site for the phosphate group of l-phosphoserine. Comparison with Escherichia coli phosphoserine aminotransferase in complex with the substrate analogue alpha-methylglutamate revealed more extensive structural changes in the case of l-phosphoserine binding. Based on the structural analysis, the flexibility of Arg328 is proposed to be critical for substrate recognition.
UTU Research Portal