A1 Refereed original research article in a scientific journal
Detection of pathogenic Streptococcus suis bacteria using magnetic glycoparticles
Authors: Pera NP, Kouki A, Haataja S, Branderhorst HM, Liskamp RMJ, Visser GM, Finne J, Pieters RJ
Publisher: ROYAL SOC CHEMISTRY
Publication year: 2010
Journal: Organic and Biomolecular Chemistry
Journal name in source: ORGANIC & BIOMOLECULAR CHEMISTRY
Journal acronym: ORG BIOMOL CHEM
Number in series: 10
Volume: 8
Issue: 10
First page : 2425
Last page: 2429
Number of pages: 5
ISSN: 1477-0520
DOI: https://doi.org/10.1039/c000819b
Abstract
Detection of the zoonotic bacterial pathogen Streptococcus suis was achieved using magnetic glycoparticles. The bacteria contain an adhesion protein for the carbohydrate sequence Gal alpha 1,4Gal. After incubation with various amounts of the pathogen, magnetic concentration and ATP detection, bacterial levels down to 10(5) cfu could be detected. Submicrometer particles were needed, since with the larger microparticles the method did not succeed.
Detection of the zoonotic bacterial pathogen Streptococcus suis was achieved using magnetic glycoparticles. The bacteria contain an adhesion protein for the carbohydrate sequence Gal alpha 1,4Gal. After incubation with various amounts of the pathogen, magnetic concentration and ATP detection, bacterial levels down to 10(5) cfu could be detected. Submicrometer particles were needed, since with the larger microparticles the method did not succeed.
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