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Detection of pathogenic Streptococcus suis bacteria using magnetic glycoparticles
Tekijät: Pera NP, Kouki A, Haataja S, Branderhorst HM, Liskamp RMJ, Visser GM, Finne J, Pieters RJ
Kustantaja: ROYAL SOC CHEMISTRY
Julkaisuvuosi: 2010
Journal: Organic and Biomolecular Chemistry
Tietokannassa oleva lehden nimi: ORGANIC & BIOMOLECULAR CHEMISTRY
Lehden akronyymi: ORG BIOMOL CHEM
Numero sarjassa: 10
Vuosikerta: 8
Numero: 10
Aloitussivu: 2425
Lopetussivu: 2429
Sivujen määrä: 5
ISSN: 1477-0520
DOI: https://doi.org/10.1039/c000819b
Tiivistelmä
Detection of the zoonotic bacterial pathogen Streptococcus suis was achieved using magnetic glycoparticles. The bacteria contain an adhesion protein for the carbohydrate sequence Gal alpha 1,4Gal. After incubation with various amounts of the pathogen, magnetic concentration and ATP detection, bacterial levels down to 10(5) cfu could be detected. Submicrometer particles were needed, since with the larger microparticles the method did not succeed.
Detection of the zoonotic bacterial pathogen Streptococcus suis was achieved using magnetic glycoparticles. The bacteria contain an adhesion protein for the carbohydrate sequence Gal alpha 1,4Gal. After incubation with various amounts of the pathogen, magnetic concentration and ATP detection, bacterial levels down to 10(5) cfu could be detected. Submicrometer particles were needed, since with the larger microparticles the method did not succeed.
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