A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Novel homogenous time-resolved fluorometric RT-PCR assays for quantification of PSA and hK2 mRNAs in blood
Tekijät: Rissanen M, Helo P, Vaananen RM, Wahlroos V, Lilja H, Nurmi M, Pettersson K, Nurmi J
Kustantaja: PERGAMON-ELSEVIER SCIENCE LTD
Julkaisuvuosi: 2007
Lehti:Clinical Biochemistry
Tietokannassa oleva lehden nimiCLINICAL BIOCHEMISTRY
Lehden akronyymi: CLIN BIOCHEM
Vuosikerta: 40
Numero: 1-2
Aloitussivu: 111
Lopetussivu: 118
Sivujen määrä: 8
ISSN: 0009-9120
DOI: https://doi.org/10.1016/j.clinbiochem.2006.10.005
Tiivistelmä
Conclusions: We developed an internally standardized, specific and reproducible real-time RT-PCR analysis method for PSA and hK2 mRNA in circulating cells in the bloodstream. Both PSA and hK2 assays are sufficiently sensitive to detect two LNCaP cells per milliliter whole blood. (c) 2006 The Canadian Society of Clinical Chemists. All rights reserved.
Conclusions: We developed an internally standardized, specific and reproducible real-time RT-PCR analysis method for PSA and hK2 mRNA in circulating cells in the bloodstream. Both PSA and hK2 assays are sufficiently sensitive to detect two LNCaP cells per milliliter whole blood. (c) 2006 The Canadian Society of Clinical Chemists. All rights reserved.
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