A1 Refereed original research article in a scientific journal
Novel homogenous time-resolved fluorometric RT-PCR assays for quantification of PSA and hK2 mRNAs in blood
Authors: Rissanen M, Helo P, Vaananen RM, Wahlroos V, Lilja H, Nurmi M, Pettersson K, Nurmi J
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Publication year: 2007
Journal:Clinical Biochemistry
Journal name in sourceCLINICAL BIOCHEMISTRY
Journal acronym: CLIN BIOCHEM
Volume: 40
Issue: 1-2
First page : 111
Last page: 118
Number of pages: 8
ISSN: 0009-9120
DOI: https://doi.org/10.1016/j.clinbiochem.2006.10.005
Abstract
Conclusions: We developed an internally standardized, specific and reproducible real-time RT-PCR analysis method for PSA and hK2 mRNA in circulating cells in the bloodstream. Both PSA and hK2 assays are sufficiently sensitive to detect two LNCaP cells per milliliter whole blood. (c) 2006 The Canadian Society of Clinical Chemists. All rights reserved.
Conclusions: We developed an internally standardized, specific and reproducible real-time RT-PCR analysis method for PSA and hK2 mRNA in circulating cells in the bloodstream. Both PSA and hK2 assays are sufficiently sensitive to detect two LNCaP cells per milliliter whole blood. (c) 2006 The Canadian Society of Clinical Chemists. All rights reserved.
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