A novel approach to solid phase chemical synthesis of oligonucleotide mRNA cap analogs

: Jemielity J, Heinonen P, Lonnberg H, Darzynkiewicz E

Publisher: TAYLOR & FRANCIS INC

: 2005

Nucleosides, Nucleotides and Nucleic Acids

: NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS

: NUCLEOS NUCLEOT NUCL

: 24

: 5-7

: 601

: 605

: 5

: 1525-7770

DOI: https://doi.org/10.1081/NCN-200061922

A novel approach for the synthesis of 5-capped 2'-O-methyloligoribonucleotides on a disulfide-tethered solid support is described. The key step of the synthesis is ZnCl2 promoted coupling of m(7) GDP imidazolide to a fully deprotected oh olignucleotide 5'-phosphate on-support. By this methodology m(7)G5' pppm(2') Apm(2') Upm(2') Ap has been prepared.

Chemical sciences