A1 Refereed original research article in a scientific journal
A novel approach to solid phase chemical synthesis of oligonucleotide mRNA cap analogs
Authors: Jemielity J, Heinonen P, Lonnberg H, Darzynkiewicz E
Publisher: TAYLOR & FRANCIS INC
Publication year: 2005
Journal:: Nucleosides, Nucleotides and Nucleic Acids
Journal name in source: NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
Journal acronym: NUCLEOS NUCLEOT NUCL
Volume: 24
Issue: 5-7
First page : 601
Last page: 605
Number of pages: 5
ISSN: 1525-7770
DOI: https://doi.org/10.1081/NCN-200061922
Abstract
A novel approach for the synthesis of 5-capped 2'-O-methyloligoribonucleotides on a disulfide-tethered solid support is described. The key step of the synthesis is ZnCl2 promoted coupling of m(7) GDP imidazolide to a fully deprotected oh olignucleotide 5'-phosphate on-support. By this methodology m(7)G5' pppm(2') Apm(2') Upm(2') Ap has been prepared.
A novel approach for the synthesis of 5-capped 2'-O-methyloligoribonucleotides on a disulfide-tethered solid support is described. The key step of the synthesis is ZnCl2 promoted coupling of m(7) GDP imidazolide to a fully deprotected oh olignucleotide 5'-phosphate on-support. By this methodology m(7)G5' pppm(2') Apm(2') Upm(2') Ap has been prepared.
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