A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Intracomplex general acid/base catalyzed cleavage of RNA phosphodiester bonds: the leaving group effect
Tekijät: Lonnberg T, Luomala M
Kustantaja: ROYAL SOC CHEMISTRY
Julkaisuvuosi: 2012
Journal: Organic and Biomolecular Chemistry
Tietokannassa oleva lehden nimi: ORGANIC & BIOMOLECULAR CHEMISTRY
Lehden akronyymi: ORG BIOMOL CHEM
Numero sarjassa: 33
Vuosikerta: 10
Numero: 33
Aloitussivu: 6785
Lopetussivu: 6791
Sivujen määrä: 7
ISSN: 1477-0520
DOI: https://doi.org/10.1039/c2ob25958c
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/1597510
The general acid/base catalyzed cleavage of a number of alkyl esters of uridine-3'-(and -5'-) phosphate has been studied by utilizing a cleaving agent, in which the catalytic moiety (a substituted 1,3,5-triazine) is tethered to an anchoring Zn-II: cyclen moiety. Around pH 7, formation of a strong ternary complex between uracil, Zn-II and cyclen brings the general acid/base catalyst close to the scissile phosphodiester linkage, resulting in rate acceleration of 1-2 orders of magnitude with the uridine-3'-phosphodiesters. Curiously, no acceleration was observed with their 5'-counterparts. A beta(lg) value of -0.7 has been determined for the general acid/base catalyzed cleavage, consistent with a proton transfer to the leaving group in the rate-limiting step.
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