A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Time-resolved fluorescence immunoassay for C-reactive protein using colloidal semiconducting nanoparticles
Tekijät: Härmä H, Toivonen J, Soini J, Hänninen P, Parak W
Julkaisuvuosi: 2011
Journal: Sensors
Tietokannassa oleva lehden nimi: Sensors
Numero sarjassa: 12
Vuosikerta: 11
Numero: 12
Aloitussivu: 11335
Lopetussivu: 11342
Sivujen määrä: 8
ISSN: 1424-8220
DOI: https://doi.org/10.3390/s111211335
Verkko-osoite: http://api.elsevier.com/content/abstract/scopus_id:84255191213
Tiivistelmä
Besides the typical short-lived fluorescence with decay times in the nanosecond range, colloidal II/VI semiconductor nanoparticles dispersed in buffer also possess a long-lived fluorescence component with decay times in the microsecond range. Here, the signal intensity of the long-lived luminescence at microsecond range is shown to increase 1,000-fold for CdTe nanoparticles in PBS buffer. This long-lived fluorescence can be conveniently employed for time-gated fluorescence detection, which allows for improved signal-to-noise ratio and thus the use of low concentrations of nanoparticles. The detection principle is demonstrated with a time-resolved fluorescence immunoassay for the detection of C-reactive protein (CRP) using CdSe-ZnS nanoparticles and green light excitation. © 2011 by the authors; licensee MDPI, Basel, Switzerland.
Besides the typical short-lived fluorescence with decay times in the nanosecond range, colloidal II/VI semiconductor nanoparticles dispersed in buffer also possess a long-lived fluorescence component with decay times in the microsecond range. Here, the signal intensity of the long-lived luminescence at microsecond range is shown to increase 1,000-fold for CdTe nanoparticles in PBS buffer. This long-lived fluorescence can be conveniently employed for time-gated fluorescence detection, which allows for improved signal-to-noise ratio and thus the use of low concentrations of nanoparticles. The detection principle is demonstrated with a time-resolved fluorescence immunoassay for the detection of C-reactive protein (CRP) using CdSe-ZnS nanoparticles and green light excitation. © 2011 by the authors; licensee MDPI, Basel, Switzerland.
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