A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Twinfilin is required for actin-dependent developmental processes in Drosophila
Tekijät: Wahlstrom G, Vartiainen M, Yamamoto L, Mattila PK, Lappalainen P, Heino TI
Kustantaja: ROCKEFELLER UNIV PRESS
Julkaisuvuosi: 2001
Journal: Journal of Cell Biology
Tietokannassa oleva lehden nimi: JOURNAL OF CELL BIOLOGY
Lehden akronyymi: J CELL BIOL
Vuosikerta: 155
Numero: 5
Aloitussivu: 787
Lopetussivu: 795
Sivujen määrä: 9
ISSN: 0021-9525
DOI: https://doi.org/10.1083/jcb.200108022
Tiivistelmä
The actin cytoskeleton is essential for cellular remodeling and many developmental and morphological processes. Twinfilin is a ubiquitous actin monomer-binding protein whose biological function has remained unclear. We discovered and cloned the Drosophila twinfilin homologue, and show that this protein is ubiquitously expressed in different tissues and developmental stages. A mutation in the twf gene leads to a number of developmental defects, including aberrant bristle morphology. This results from uncontrolled polymerization of actin filaments and misori-entation of actin bundles in developing bristles. In wildtype bristles, twinfilin localizes diffusively to cytoplasm and to the ends of actin bundles, and may therefore be involved in localization of actin monomers in cells. We also show that twinfilin and the ADF/cofilin encoding gene twinstar interact genetically in bristle morphogenesis. These results demonstrate that the accurate regulation of size and dynamics of the actin monomer pool by twinfilin is essential for a number of actin-dependent developmental processes in multicellular eukaryotes.
The actin cytoskeleton is essential for cellular remodeling and many developmental and morphological processes. Twinfilin is a ubiquitous actin monomer-binding protein whose biological function has remained unclear. We discovered and cloned the Drosophila twinfilin homologue, and show that this protein is ubiquitously expressed in different tissues and developmental stages. A mutation in the twf gene leads to a number of developmental defects, including aberrant bristle morphology. This results from uncontrolled polymerization of actin filaments and misori-entation of actin bundles in developing bristles. In wildtype bristles, twinfilin localizes diffusively to cytoplasm and to the ends of actin bundles, and may therefore be involved in localization of actin monomers in cells. We also show that twinfilin and the ADF/cofilin encoding gene twinstar interact genetically in bristle morphogenesis. These results demonstrate that the accurate regulation of size and dynamics of the actin monomer pool by twinfilin is essential for a number of actin-dependent developmental processes in multicellular eukaryotes.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
Turun yliopiston Tutkimustietojärjestelmän portaali