Cutin composition of selected northern berries and seeds

: Jarvinen R, Kaimainen M, Kallio H

Publisher: ELSEVIER SCI LTD

: 2010

: Food Chemistry

: FOOD CHEMISTRY

: FOOD CHEM

: 1

: 122

: 1

: 137

: 144

: 8

: 0308-8146

DOI: https://doi.org/10.1016/j.foodchem.2010.02.030

Monomeric compositions of the extractive-free cuticular polymer fractions of seven berries - rosehip (Rosa rugosa), black chokeberry (Aronia melanocarpa), strawberry (Fragaria x ananassa), raspberry (Rubus idaeus), cloudberry (Rubus chamaemorus), crowberry (Empetrum nigrum) and rowanberry (Sorbus aucuparia) - as well as the seeds from three berries - cloudberry, sea buckthorn (Hippophae rhamnoides) and blackcurrant (Ribes nigrum) - were investigated. Depolymerisation of the cutin polymer was carried out using NaOMe-catalysed methanolysis and the composition of TMS-derivatised monomers was determined by GC using FID and MS detection. Two depolymerisation techniques were compared: 1.0 M NaOMe at ambient temperature and 1.3 M NaOMe under reflux conditions. The degree of depolymerisation of the cuticular membrane ranged from 2% (strawberry) to 84% (rosehip) for the CHCl(3)-soluble cutin monomers. Depolymerisation of seed samples was low (1-3%). The predominant monomers were C(16) and C(18) omega-hydroxy acids with mid-chain functionalities, mainly epoxy and hydroxy groups, but suberin-like alpha,omega-dicarboxylic acids, with mid-chain hydroxyl groups and C(15) chain length monomers, were also found. Seed cutin monomers differed from the corresponding berry skin monomers. (C) 2010 Elsevier Ltd. All rights reserved.