A1 Refereed original research article in a scientific journal
Cutin composition of selected northern berries and seeds
Authors: Jarvinen R, Kaimainen M, Kallio H
Publisher: ELSEVIER SCI LTD
Publication year: 2010
Journal: Food Chemistry
Journal name in source: FOOD CHEMISTRY
Journal acronym: FOOD CHEM
Number in series: 1
Volume: 122
Issue: 1
First page : 137
Last page: 144
Number of pages: 8
ISSN: 0308-8146
DOI: https://doi.org/10.1016/j.foodchem.2010.02.030
Abstract
Monomeric compositions of the extractive-free cuticular polymer fractions of seven berries - rosehip (Rosa rugosa), black chokeberry (Aronia melanocarpa), strawberry (Fragaria x ananassa), raspberry (Rubus idaeus), cloudberry (Rubus chamaemorus), crowberry (Empetrum nigrum) and rowanberry (Sorbus aucuparia) - as well as the seeds from three berries - cloudberry, sea buckthorn (Hippophae rhamnoides) and blackcurrant (Ribes nigrum) - were investigated. Depolymerisation of the cutin polymer was carried out using NaOMe-catalysed methanolysis and the composition of TMS-derivatised monomers was determined by GC using FID and MS detection. Two depolymerisation techniques were compared: 1.0 M NaOMe at ambient temperature and 1.3 M NaOMe under reflux conditions. The degree of depolymerisation of the cuticular membrane ranged from 2% (strawberry) to 84% (rosehip) for the CHCl(3)-soluble cutin monomers. Depolymerisation of seed samples was low (1-3%). The predominant monomers were C(16) and C(18) omega-hydroxy acids with mid-chain functionalities, mainly epoxy and hydroxy groups, but suberin-like alpha,omega-dicarboxylic acids, with mid-chain hydroxyl groups and C(15) chain length monomers, were also found. Seed cutin monomers differed from the corresponding berry skin monomers. (C) 2010 Elsevier Ltd. All rights reserved.
Monomeric compositions of the extractive-free cuticular polymer fractions of seven berries - rosehip (Rosa rugosa), black chokeberry (Aronia melanocarpa), strawberry (Fragaria x ananassa), raspberry (Rubus idaeus), cloudberry (Rubus chamaemorus), crowberry (Empetrum nigrum) and rowanberry (Sorbus aucuparia) - as well as the seeds from three berries - cloudberry, sea buckthorn (Hippophae rhamnoides) and blackcurrant (Ribes nigrum) - were investigated. Depolymerisation of the cutin polymer was carried out using NaOMe-catalysed methanolysis and the composition of TMS-derivatised monomers was determined by GC using FID and MS detection. Two depolymerisation techniques were compared: 1.0 M NaOMe at ambient temperature and 1.3 M NaOMe under reflux conditions. The degree of depolymerisation of the cuticular membrane ranged from 2% (strawberry) to 84% (rosehip) for the CHCl(3)-soluble cutin monomers. Depolymerisation of seed samples was low (1-3%). The predominant monomers were C(16) and C(18) omega-hydroxy acids with mid-chain functionalities, mainly epoxy and hydroxy groups, but suberin-like alpha,omega-dicarboxylic acids, with mid-chain hydroxyl groups and C(15) chain length monomers, were also found. Seed cutin monomers differed from the corresponding berry skin monomers. (C) 2010 Elsevier Ltd. All rights reserved.
Keywords:
Berry, Cutin, Epoxy fatty acids, Hydroxy fatty acids, Mass spectrometry, Seed
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