Sequence-selective cleavage of oligoribonucleotides

: Niittymaki T, Lonnberg H

: 2005

Collection Symposium Series

Chemistry of Nucleic Acid Components

: COLL SYMPOS SERIES

: 7

: 443

: 444

: 2

: 80-86241-25-4

2'-O-Methyl oligoribonucleotides bearing a 1,5,9-triazacyclododec-3-yl group tethered either to the 3'-terminus of an oligonucleotide or to an intrachain abasic sugar unit have been shown to cleave in slight excess of Zn2+, ions complementary oligoribonucleotides. The cleavage obeys first-order kinetics and exhibits turnover. The accelaration compared to the monomeric Zn2+ 1,5,9-triazacyclododecane chelate is more than 100-fold for the 3'-tethered conjugates and approximately 50-fold for the intrachain conjugates.

Chemical sciences