Fish scales and SNP chips: SNP genotyping and allele frequency estimation in individual and pooled DNA from historical samples of Atlantic salmon (Salmo salar)



: Johnston SE, Lindqvist M, Niemela E, Orell P, Erkinaro J, Kent MP, Lien S, Vaha JP, Vasemagi A, Primmer CR

Publisher: BIOMED CENTRAL LTD

: 2013

: BMC Genomics

: BMC GENOMICS

: BMC GENOMICS

: 439

: 14

: 439

: 13

: 1471-2164

DOI: https://doi.org/10.1186/1471-2164-14-439


Conclusions: SNP genotyping was highly successful in degraded DNA samples, paving the way for the use of degraded samples in SNP genotyping projects. DNA pooling provides the potential for large scale population genetic studies with fewer assays, provided enough reference individuals are also genotyped and DNA quality is properly assessed beforehand. We provide recommendations for future studies intending to conduct high-throughput SNP genotyping and allele frequency estimation in historical samples.



Last updated on 2024-26-11 at 20:44