A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Fish scales and SNP chips: SNP genotyping and allele frequency estimation in individual and pooled DNA from historical samples of Atlantic salmon (Salmo salar)
Tekijät: Johnston SE, Lindqvist M, Niemela E, Orell P, Erkinaro J, Kent MP, Lien S, Vaha JP, Vasemagi A, Primmer CR
Kustantaja: BIOMED CENTRAL LTD
Julkaisuvuosi: 2013
Journal: BMC Genomics
Tietokannassa oleva lehden nimi: BMC GENOMICS
Lehden akronyymi: BMC GENOMICS
Numero sarjassa: 439
Vuosikerta: 14
Numero: 439
Sivujen määrä: 13
ISSN: 1471-2164
DOI: https://doi.org/10.1186/1471-2164-14-439
Tiivistelmä
Conclusions: SNP genotyping was highly successful in degraded DNA samples, paving the way for the use of degraded samples in SNP genotyping projects. DNA pooling provides the potential for large scale population genetic studies with fewer assays, provided enough reference individuals are also genotyped and DNA quality is properly assessed beforehand. We provide recommendations for future studies intending to conduct high-throughput SNP genotyping and allele frequency estimation in historical samples.
Conclusions: SNP genotyping was highly successful in degraded DNA samples, paving the way for the use of degraded samples in SNP genotyping projects. DNA pooling provides the potential for large scale population genetic studies with fewer assays, provided enough reference individuals are also genotyped and DNA quality is properly assessed beforehand. We provide recommendations for future studies intending to conduct high-throughput SNP genotyping and allele frequency estimation in historical samples.
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