Juha Kurkela
PhD
jhmkur@utu.fi Itäinen Pitkäkatu 4 C Turku : 6028 Office hours 10:00-14:00 |
Transcription, Molecular biology, Cyanobacteria, Photosynthesis
I'm Postdoctoral researcher in Molecular Plant Biology unit in a project lead by Taina Tyystjärvi - Bioproduction in cyanobacteria. Already since high school I have had a great
interest towards biology, which led me in 2010 to University of Turku in the
department of biology. During my years in the university I have deepened
the knowledge in biology and after second year of bachelor studies I found how
fascinating photosynthesis and microbiology is. That is how I found my area of interest and pursued to work with photosynthetic prokaryotes, cyanobacteria. My scientific career started as a summer worker in molecular plant biology unit in "Gene
regulation in cyanobacteria" project. In the
project I learned how to do basic lab work and numerous techniques from the
field of molecular biology, photosynthesis and microbiology. Later I learned to
design experiments and to write scientific articles. Meanwhile I got bachelors degree and masters degree and from there I continued
working as a doctoral student. I completed my doctoral studies in the doctoral programme of molecular life sciences and defended my thesis: "The biological role of omega subunit of cyanobacterial RNA polymerase" in 2020.
Being very social person,
I have also been good in making contacts with other researches. Organizing
things is one of my strengths i.e. making my time usage as efficient as
possible. That’s why it has been possible to me to work and study simultaneously.
When adding cycling, badminton and doing exercise at the local gym to the
equation, I think my life is well balanced.
My main
research topic is the RNA polymerase of cyanobacteria and model organism is Synechocystis sp PCC 6803
(Synechocystis). Cyanobacteria have only one type of RNA polymerase which makes
all the RNA species. This RNA polymerase is a multisubunit complex consisting
from two α subunits, β, β´, γ
and ω subunits. Additional factor, called a σ factor, is recruited to the core
complex (2αββ´γω) when transcription initiation takes place. σ factor
recognizes the promoter sequence and regulates which set of genes are
transcribed. There are nine different σ factors in Synechocystis and my research considers group one σ factor (SigA)
and group two σ factors: SigB, SigC, SigD and SigE. SigA is responsible of the
expression of household genes, whereas group two σ factors are required when
acclimating to stress. In my doctoral work the gene encoding the ω subunit
(rpoZ) has been disrupted from Synechocystis
and the ω-less strain is used to find out the role of the ω subunit. I have
found out that ω subunit is not essential for cyanobacteria, but it affects
recruitment of σ factors. ω-less strain is also heat sensitive and more surprisingly
sensitive towards high CO2 concentrations. Currently I’m studying
novel carbon signaling pathway.
- Engineering RNA polymerase to construct biotechnological host strains of cyanobacteria (2024)
- Physiologia Plantarum
- Inorganic carbon sensing and signalling in cyanobacteria (2024)
- Physiologia Plantarum
- The genome sequence of Synechocystis sp. PCC 6803 substrain GT-T and its implications for the evolution of PCC 6803 substrains (2023)
- FEBS Open Bio
- Roles of Close Homologues SigB and SigD in Heat and High Light Acclimation of the Cyanobacterium Synechocystis sp. PCC 6803 (2022)
- Life
- Revealing secrets of the enigmatic omega subunit of bacterial RNA polymerase (2020)
- Molecular Microbiology
- Testing the Potential of Regulatory Sigma Factor Mutants for Wastewater Purification or Bioreactor Run in High Light (2020)
- Current Microbiology
- The biological role of omega subunit of cyanobacterial RNA polymerase (2020) Kurkela Juha
- Inactivation of group 2 sigma factors upregulates production of transcription and translation machineries in the cyanobacterium Synechocystis sp PCC 6803 (2018)
- Scientific Reports
- Acclimation to High CO2 Requires the omega Subunit of the RNA Polymerase in Synechocystis (2017)
- Plant Physiology
- In vivo recruitment analysis and a mutant strain without any group 2 sigma factor reveal roles of different sigma factors in cyanobacteria (2016)
- Molecular Microbiology
- Roles of Group 2 Sigma Factors in Acclimation of the Cyanobacterium Synechocystis sp PCC 6803 to Nitrogen Deficiency (2016)
- Plant and Cell Physiology
- Oxidative stress and photoinhibition can be separated in the cyanobacterium Synechocystis sp. PCC 6803 (2014)
- BBA - Bioenergetics
- The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria (2014)
- Nucleic Acids Research
- The ω subunit of RNA polymerase is essential for thermal acclimation of cyanobacterium Synechocystis sp. PCC 6803 (2014)
- PLoS ONE
- Group 2 sigma factor mutant ΔsigCDE of the cyanobacterium Synechocystis sp. PCC 6803 reveals functionality of both carotenoids and flavodiiron proteins in photoprotection of Photosystem II (2013)
- Plant and Cell Physiology