Hiroaki Fujii
Ph.D
hiroaki.fujii@utu.fi +358 29 450 4208 +358 50 574 3891 Tykistökatu 6 Turku ORCID identifier: https://orcid.org/0000-0002-0013-5891 |
Molecular Plant Biology; Abiotic stress signalling;
2003 PhD, Kyoto University, Japan
2003-2011 Postdoc, University of Arizona, USA
University of California, Riverside, USA
KAUST, Saudi Arabia
2012-present Junior Group Leader, University of Turku
Analysis of
osmotic stress signaling in plants
Salinity, drought, and low temperature cause osmotic
stress in plants and activate signalling pathways that regulate plant
responses. The understanding of plant signalling pathways that respond to
osmotic stress remains limited in spite of its importance for both basic
biology and agriculture. Our previous study revealed that the SNF1-related protein kinase 2 family
(SnRK2s) plays a central role in osmotic stress responses. In our project with model plant Arabidopsis thaliana, we will use genetics,
and biochemical techniques
to examine molecular interactions between SnRK2s and other signaling components
in order to clarify the nature of osmotic stress pathways.
Several
literatures and our preliminary results suggest that osmotic stress induces
posttranslational modification of SnRK2s. We will further characterize this
modification to determine the activation mechanism of SnRK2s in the osmotic
stress pathway. We will also determine the function of proteins that regulate
SnRK2s in the osmotic stress pathway; these proteins have been identified in
yeast two-hybrid screening. Because some
key players in osmotic stress signaling have yet to be identified, other
screenings will also be performed.
Through these projects, we expect to generate new knowledge regarding the regulatory
mechanism of SnRK2s and proteins downstream of SnRK2s, which play central roles
in osmotic stress signaling.
KABI 5061 Literature in
Plant Biology
KABI4006
Plant acclimation and Development (together with other lecturers)
- Arabidopsis mutant deficient in 3 abscisic acid-activated protein kinases reveals critical roles in growth, reproduction, and stress (2009)
- Proceedings of the National Academy of Sciences of the United States of America
(A1 Refereed original research article in a scientific journal) - A Rice Kinase-Protein Interaction Map (2009)
- Plant Physiology
(A1 Refereed original research article in a scientific journal) - In vitro reconstitution of an abscisic acid signalling pathway (2009)
- Nature
(A1 Refereed original research article in a scientific journal) - Protein-Protein Interactions of Tandem Affinity Purified Protein Kinases from Rice (2009)
- PLoS ONE
(A1 Refereed original research article in a scientific journal) - SCREAM/ICE1 and SCREAM2 specify three cell-state transitional steps leading to Arabidopsis stomatal differentiation (2008)
- Plant Cell
(A1 Refereed original research article in a scientific journal) - Identification of two protein kinases required for abscisic acid regulation of seed germination, root growth, and gene expression in Arabidopsis (2007)
- Plant Cell
(A1 Refereed original research article in a scientific journal) - SOS2 promotes salt tolerance in part by interacting with the vacuolar H+-ATPase and upregulating its transport activity (2007)
- Molecular and Cellular Biology
(A1 Refereed original research article in a scientific journal) - The structure of the C-terminal domain of the protein kinase AtSOS2 bound to the calcium sensor AtSOS3 (2007)
- Molecular Cell
(A1 Refereed original research article in a scientific journal) - A R2R3 type MYB transcription factor is involved in the cold regulation of CBF genes and in acquired freezing tolerance (2006)
- Journal of Biological Chemistry
(A1 Refereed original research article in a scientific journal) - A miRNA involved in phosphate-starvation response in Arabidopsis (2005)
- Current Biology
(A1 Refereed original research article in a scientific journal) - Calcineurin regulates induction of late phase of cerebellar long-term depression in rat cultured Purkinje neurons (2002)
- European Journal of Neuroscience
(A1 Refereed original research article in a scientific journal) - Requirement for mitogen-activated protein kinase in cerebellar long term depression (1999)
- Journal of Biological Chemistry
(A1 Refereed original research article in a scientific journal)