Hiroaki Fujii
Ph.D
hiroaki.fujii@utu.fi +358 29 450 4208 +358 50 574 3891 Tykistökatu 6 Turku ORCID identifier: https://orcid.org/0000-0002-0013-5891 |
Molecular Plant Biology; Abiotic stress signalling;
2003 PhD, Kyoto University, Japan
2003-2011 Postdoc, University of Arizona, USA
University of California, Riverside, USA
KAUST, Saudi Arabia
2012-present Junior Group Leader, University of Turku
Analysis of
osmotic stress signaling in plants
Salinity, drought, and low temperature cause osmotic
stress in plants and activate signalling pathways that regulate plant
responses. The understanding of plant signalling pathways that respond to
osmotic stress remains limited in spite of its importance for both basic
biology and agriculture. Our previous study revealed that the SNF1-related protein kinase 2 family
(SnRK2s) plays a central role in osmotic stress responses. In our project with model plant Arabidopsis thaliana, we will use genetics,
and biochemical techniques
to examine molecular interactions between SnRK2s and other signaling components
in order to clarify the nature of osmotic stress pathways.
Several
literatures and our preliminary results suggest that osmotic stress induces
posttranslational modification of SnRK2s. We will further characterize this
modification to determine the activation mechanism of SnRK2s in the osmotic
stress pathway. We will also determine the function of proteins that regulate
SnRK2s in the osmotic stress pathway; these proteins have been identified in
yeast two-hybrid screening. Because some
key players in osmotic stress signaling have yet to be identified, other
screenings will also be performed.
Through these projects, we expect to generate new knowledge regarding the regulatory
mechanism of SnRK2s and proteins downstream of SnRK2s, which play central roles
in osmotic stress signaling.
KABI 5061 Literature in
Plant Biology
KABI4006
Plant acclimation and Development (together with other lecturers)
- Defining the heterogeneous composition of Arabidopsis thylakoid membrane (2025)
- Plant Journal
(A1 Refereed original research article in a scientific journal) - Temperature-dependent conformational changes in Arabidopsis DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN 2A (2025)
- Acta Physiologiae Plantarum
(A1 Refereed original research article in a scientific journal) - In Vitro Kinase Assay with Recombinant SnRK2s : An Example for Assaying Stress-Responsive Kinases in Plants (2024)
- Methods in Molecular Biology
(A3 Refereed book chapter or chapter in a compilation book) - Chemical activation of Arabidopsis SnRK2.6 by pladienolide B (2021)
- Plant Signaling and Behavior
(A1 Refereed original research article in a scientific journal) - EARLY RESPONSE TO DEHYDRATION 7 Remodels Cell Membrane Lipid Composition During Cold Stress in Arabidopsis (2021)
- Plant and Cell Physiology
(A1 Refereed original research article in a scientific journal) - Arabidopsis KIN gamma subunit 1 has a potential to regulate activity of sucrose nonfermenting 1-related protein kinase 2s (SnRK2s) in vitro (2019)
- Biologia Plantarum
(A1 Refereed original research article in a scientific journal) - Upstream kinases of plant SnRKs are involved in salt stress tolerance (2018)
- Plant Journal
(A1 Refereed original research article in a scientific journal) - Genome sequencing and population genomic analyses provide insights into the adaptive landscape of silver birch. (2017)
- Nature Genetics
(A1 Refereed original research article in a scientific journal) - Pre-mRNA splicing repression triggers abiotic stress signaling in plants (2017)
- Plant Journal
(A1 Refereed original research article in a scientific journal) - Dissecting Abscisic Acid Signaling Pathways Involved in Cuticle Formation (2016)
- Molecular Plant
(A1 Refereed original research article in a scientific journal) - Integration of photosynthesis, development and stress as an opportunity for plant biology. (2015)
- New Phytologist
(A1 Refereed original research article in a scientific journal) - ABA signaling is necessary but not sufficient for RD29B transcriptional memory during successive dehydration stresses in Arabidopsis thaliana. (2014)
- Plant Journal
(A1 Refereed original research article in a scientific journal) - Abscisic Acid Implication in Plant Growth and Stress Responses (2014) Phytohormones: A Window to Metabolism, Signaling and Biotechnological Applications Hiroaki Fujii
(A3 Refereed book chapter or chapter in a compilation book) - GSK3-like kinases positively modulate abscisic acid signaling through phosphorylating subgroup III SnRK2s in Arabidopsis. (2014)
- Proceedings of the National Academy of Sciences of the United States of America
(A1 Refereed original research article in a scientific journal) - Osmotic stress signaling via protein kinases (2012)
- Cellular and Molecular Life Sciences
(A2 Refereed review article in a scientific journal ) - Activation of the plasma membrane Na/H antiporter Salt-Overly-Sensitive 1 (SOS1) by phosphorylation of an auto-inhibitory C-terminal domain (2011)
- Proceedings of the National Academy of Sciences of the United States of America
(A1 Refereed original research article in a scientific journal) - Arabidopsis decuple mutant reveals the importance of SnRK2 kinases in osmotic stress responses in vivo (2011)
- Proceedings of the National Academy of Sciences of the United States of America
(A1 Refereed original research article in a scientific journal) - Abscisic Acid Inhibits Type 2C Protein Phosphatases via the PYR/PYL Family of START Proteins (2009)
- Science
(A1 Refereed original research article in a scientific journal) - A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors (2009)
- Nature
(A1 Refereed original research article in a scientific journal) - An Autophosphorylation Site of the Protein Kinase SOS2 Is Important for Salt Tolerance in Arabidopsis (2009)
- Molecular Plant
(A1 Refereed original research article in a scientific journal)