Anton Zavialov
PhD, Head of Laboratory
anton.zavialov@utu.fi +358 29 450 3211 +358 50 328 6112 Arcanuminkuja 1 Turku ORCID-tunniste: https://orcid.org/orcid.org/0000-0001-6191-5931 |
Structural biology and structure-based drug design; X-ray crystallography; Cryo-electron microscopy; Microbiology and cell biology; Biochemistry; Protein chemistry; Protein engineering; Molecular chaperones; Secretion; Assembly.
Bacterial attachment, biofilm formation, and host tissue invasion. Cell-signalling mechanism of adenosine deaminase growth factor ADA2.
We work in the field of structural biology and structure-based drug design. Our main methods are x-ray crystallography and cryo-electron microscopy. The main focus of our research is the unraveling of molecular mechanisms governing the interplay between microbial pathogens and their hosts, and the exploitation of these finding in medicine. We are particularly interested in host-pathogen interactions during the early steps of infection: bacterial attachment and biofilm formation mediated by fimbrial adhesins and host tissue invasion mediated by the Type III secretion system. Blocking these steps could prevent infections caused by antibiotic-resistant pathogens. Another line of our research explores the intricate cell signaling mechanism of the novel growth factor-enzyme, adenosine deaminase type 2 (ADA2). This study paves the way for the development of novel means to treat blood cancers and vascular disorders, including a genetic disease called deficiency of ADA2 (DADA2). In addition, we develop a novel ADA-based immuno-oncological therapy of advanced cancers.
- Csu pili dependent biofilm formation and virulence of Acinetobacter baumannii (2023)
- npj biofilms and microbiomes
(A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä ) - Archaic chaperone-usher pili self-secrete into superelastic zigzag springs (2022)
- Nature
(A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä ) - ELISA based assays to measure adenosine deaminases concentration in serum and saliva for the diagnosis of ADA2 deficiency and cancer (2022)
- Frontiers in Immunology
(A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä ) - Comparison of kinetic and enzymatic properties of intracellular phosphoserine aminotransferases from alkaliphilic and neutralophilic bacteria (2020)
- Open Chemistry
(A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä ) - A new human challenge model for testing heat-stable toxin-based vaccine candidates for enterotoxigenic Escherichia coli diarrhea - dose optimization, clinical outcomes, and CD4+T cell responses (2019)
- PLoS Neglected Tropical Diseases
(A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä ) - Archaic and alternative chaperones preserve pilin folding energy by providing incomplete structural information (2018)
- Journal of Biological Chemistry
(A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä ) - Heterologous Complementation Studies With the YscX and YscY Protein Families Reveals a Specificity for Yersinia pseudotuberculosis Type III Secretion (2018)
- Frontiers in Cellular and Infection Microbiology
(A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä ) - Secreted bacterial adenosine deaminase is an evolutionary precursor of adenosine deaminase growth factor (2018)
- Molecular Biology and Evolution
(A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä ) - Structural basis for Acinetobacter baumannii biofilm formation (2018)
- Proceedings of the National Academy of Sciences of the United States of America
(A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä ) - Human adenosine deaminases ADA1 and ADA2 bind to different subsets of immune cells (2017)
- Cellular and Molecular Life Sciences
(A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä ) - Methylation, crystallization and SAD phasing of the Csu pilus CsuC-CsuE chaperone-adhesin subunit pre-assembly complex from Acinetobacter baumannii (2017)
- Acta Crystallographica Section F: Structural Biology Communications
(A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä ) - Structural basis for Myf and Psa fimbriae-mediated tropism of pathogenic strains of Yersinia for host tissues (2016)
- Molecular Microbiology
(A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä ) - YopN and TyeA Hydrophobic Contacts Required for Regulating Ysc-Yop Type III Secretion Activity by Yersinia pseudotuberculosis (2016)
- Frontiers in Cellular and Infection Microbiology
(A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä ) - Crystallization and preliminary X-ray diffraction analysis of the Csu pili CsuC-CsuA/B chaperone-major subunit pre-assembly complex from Acinetobacter baumannii (2015)
- Acta Crystallographica Section F: Structural Biology and Crystallization Communications
(A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä ) - Dermatologic Features of ADA2 Deficiency in Cutaneous Polyarteritis Nodosa (2015)
- JAMA Dermatology
(A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä ) - Structural Insight into Archaic and Alternative Chaperone-Usher Pathways Reveals a Novel Mechanism of Pilus Biogenesis (2015)
- PLoS Pathogens
(A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä ) - Early- Onset Stroke and Vasculopathy Associated with Mutations in ADA2 (2014)
- New England Journal of Medicine
(A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä ) - Structural Insight into Host Recognition by Aggregative Adherence Fimbriae of Enteroaggregative Escherichia coli (2014)
- PLoS Pathogens
(A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä ) - Crystallization and sulfur SAD phasing of AggA, the major subunit of aggregative adherence fimbriae type I from the Escherchia coli strain that caused an outbreak of haemolytic-uraemic syndrome in Germany (2013)
- Acta Crystallographica Section F: Structural Biology and Crystallization Communications
(A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä ) - Allosteric Mechanism Controls Traffic in the Chaperone/Usher Pathway (2012)
- Structure
(A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä )