A1 Journal article – refereed
Expression, purification and crystallization of a collagen-binding fragment of Yersinia adhesin YadA
List of Authors: Nummelin H, El Tahir Y, Ollikka P, Skurnik M, Goldman A
Publication year: 2002
Volume number: 58
Abstract
A recombinant form of a collagen-binding fragment of Yersinia
enterocolitica serotype O:3 adhesin YadA with an N-terminal
polyhistidine affinity tag has been produced in Escherichia coli,
purified and crystallized using the sitting-drop vapour-diffusion
technique. Crystals belong to the trigonal space group R3, with
unit-cell parameters a = b = 67.05, c = 221.95 A, and diffract to 1.55 A
resolution on a synchrotron-radiation source.