A1 Refereed original research article in a scientific journal

Expression, purification and crystallization of a collagen-binding fragment of Yersinia adhesin YadA



Authors: Nummelin H, El Tahir Y, Ollikka P, Skurnik M, Goldman A

Publication year: 2002

Journal: Acta crystallographica section D : structural biology

Volume: 58

First page : 1042

Last page: 1044

Number of pages: 3

ISSN: 0907-4449

DOI: https://doi.org/10.1107/S0907444902005231


Abstract

A recombinant form of a collagen-binding fragment of Yersinia
enterocolitica serotype O:3 adhesin YadA with an N-terminal
polyhistidine affinity tag has been produced in Escherichia coli,
purified and crystallized using the sitting-drop vapour-diffusion
technique. Crystals belong to the trigonal space group R3, with
unit-cell parameters a = b = 67.05, c = 221.95 A, and diffract to 1.55 A
resolution on a synchrotron-radiation source.



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