A1 Journal article – refereed
Detection of circulating Yersinia-immunoglobulin complexes by enzyme immunoassay (EIA).

List of Authors: Lahesmaa-Rantala R, Granfors K, Toivanen A
Publication year: 1986
Journal: Journal of Immunological Methods
Journal name in source: Journal of immunological methods
Journal acronym: J Immunol Methods
Volume number: 89
Issue number: 2
ISSN: 0022-1759

An enzyme immunoassay (EIA) was developed for the detection of Yersinia-immunoglobulin complexes of known Ig class. Immune complexes (ICs) were attached to polystyrene microtiter plates by rabbit anti-human immunoglobulins, and the existence of Yersinia enterocolitica O:3 antigens was demonstrated using Fab fragments of alkaline phosphatase (AP)-conjugated antibody against the same serotype. Simultaneous binding of Yersinia antigens and immunoglobulins was a prerequisite for the detection of ICs. The method will be valuable for research into the immunopathogenetic mechanisms leading to reactive arthritis after Yersinia infection.

Last updated on 2019-21-08 at 21:45