A1 Refereed original research article in a scientific journal

Fish scales and SNP chips: SNP genotyping and allele frequency estimation in individual and pooled DNA from historical samples of Atlantic salmon (Salmo salar)




AuthorsJohnston SE, Lindqvist M, Niemela E, Orell P, Erkinaro J, Kent MP, Lien S, Vaha JP, Vasemagi A, Primmer CR

PublisherBIOMED CENTRAL LTD

Publication year2013

JournalBMC Genomics

Journal name in sourceBMC GENOMICS

Journal acronymBMC GENOMICS

Number in series439

Volume14

Issue439

Number of pages13

ISSN1471-2164

DOIhttps://doi.org/10.1186/1471-2164-14-439


Abstract
Conclusions: SNP genotyping was highly successful in degraded DNA samples, paving the way for the use of degraded samples in SNP genotyping projects. DNA pooling provides the potential for large scale population genetic studies with fewer assays, provided enough reference individuals are also genotyped and DNA quality is properly assessed beforehand. We provide recommendations for future studies intending to conduct high-throughput SNP genotyping and allele frequency estimation in historical samples.



Last updated on 2024-26-11 at 20:44