A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Synthesis of fluorescent oligosaccharides for covalent attachment to living cells
Tekijät: Gahmberg CG, Tolvanen M
Julkaisuvuosi: 1988
Journal: Analytical Biochemistry
Tietokannassa oleva lehden nimi: Analytical biochemistry
Lehden akronyymi: Anal Biochem
Vuosikerta: 170
Numero: 2
Aloitussivu: 520
Lopetussivu: 7
ISSN: 0003-2697
DOI: https://doi.org/10.1016/0003-2697(88)90667-7
Tiivistelmä
Asparagine-linked oligosaccharides were liberated from glycoproteins by hydrazinolysis. The treatment resulted in de-N-acetylation of the amino sugars. After isolation of the oligosaccharides free amino groups were labeled with fluorescein isothiocyanate and remaining amino groups reacetylated. The fluorescent oligosaccharides were used to label living cells. They were converted to hydrazine derivatives and covalently attached to cell surface oligosaccharides, which had been treated with periodate or neuraminidase and galactose oxidase. This enabled the visualization of the attached oligosaccharides at the external aspect of the plasma membrane by fluorescence microscopy.
Asparagine-linked oligosaccharides were liberated from glycoproteins by hydrazinolysis. The treatment resulted in de-N-acetylation of the amino sugars. After isolation of the oligosaccharides free amino groups were labeled with fluorescein isothiocyanate and remaining amino groups reacetylated. The fluorescent oligosaccharides were used to label living cells. They were converted to hydrazine derivatives and covalently attached to cell surface oligosaccharides, which had been treated with periodate or neuraminidase and galactose oxidase. This enabled the visualization of the attached oligosaccharides at the external aspect of the plasma membrane by fluorescence microscopy.
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