A1 Refereed original research article in a scientific journal
Pt-grown carbon nanofibers for enzymatic glutamate biosensors and assessment of their biocompatibility
Authors: Isoaho Noora, Peltola Emilia, Sainio Sami, Koskinen Jari, Laurila Tomi
Publisher: ROYAL SOC CHEMISTRY
Publication year: 2018
Journal: RSC Advances
Journal name in source: RSC ADVANCES
Journal acronym: RSC ADV
Volume: 8
Issue: 62
First page : 35802
Last page: 35812
Number of pages: 11
ISSN: 2046-2069
DOI: https://doi.org/10.1039/c8ra07766e
Abstract
Application-specific carbon nanofibers grown from Pt-catalyst layers have been shown to be a promising material for biosensor development. Here we demonstrate immobilization of glutamate oxidase on them and their use for amperometric detection of glutamate at two different potentials. At -0.15 V vs. Ag/AgCl at concentrations higher than 100 mu M the oxygen reduction reaction severely interferes with the enzymatic production of H2O2 and consequently affects the detection of glutamate. On the other hand, at 0.6 V vs. Ag/AgCl enzyme saturation starts to affect the measurement above a glutamate concentration of 100 mu M. Moreover, we suggest here that glutamate itself might foul Pt surfaces to some degree, which should be taken into account when designing Pt-based sensors operating at high anodic potentials. Finally, the Pt-grown and Ni-grown carbon nanofibers were shown to be biocompatible. However, the cells on Pt-grown carbon nanofibers had different morphology and formation of filopodia compared to those on Ni-grown carbon nanofibers. The effect was expected to be caused rather by the different fiber dimensions between the samples than the catalyst metal itself. Further experiments are required to find the optimal dimensions of CNFs for biological purposes.
Application-specific carbon nanofibers grown from Pt-catalyst layers have been shown to be a promising material for biosensor development. Here we demonstrate immobilization of glutamate oxidase on them and their use for amperometric detection of glutamate at two different potentials. At -0.15 V vs. Ag/AgCl at concentrations higher than 100 mu M the oxygen reduction reaction severely interferes with the enzymatic production of H2O2 and consequently affects the detection of glutamate. On the other hand, at 0.6 V vs. Ag/AgCl enzyme saturation starts to affect the measurement above a glutamate concentration of 100 mu M. Moreover, we suggest here that glutamate itself might foul Pt surfaces to some degree, which should be taken into account when designing Pt-based sensors operating at high anodic potentials. Finally, the Pt-grown and Ni-grown carbon nanofibers were shown to be biocompatible. However, the cells on Pt-grown carbon nanofibers had different morphology and formation of filopodia compared to those on Ni-grown carbon nanofibers. The effect was expected to be caused rather by the different fiber dimensions between the samples than the catalyst metal itself. Further experiments are required to find the optimal dimensions of CNFs for biological purposes.
UTU Research Portal