A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
MISpheroID: a knowledgebase and transparency tool for minimum information in spheroid identity
Tekijät: Peirsman Arne, Blondeel Eva, Ahmed Tasdiq, Anckaert Jasper, Audenaert Dominique, Boterberg Tom, Buzas Krisztina, Carragher Neil, Castellani Gastone, Castro Flavia, Dangles-Marie Virginie, Dawson John, De Tullio Pascal, De Vlieghere Elly, Dedeyne Sándor, Depypere Herman, Diosdi Akos, Dmitriev Ruslan I, Dolznig Helmut, Fischer Suzanne, Gespach Christian, Goossens Vera, Heino Jyrki, Hendrix An, Horvath Peter, Kunz-Schughart Leoni A., Maes Sebastiaan, Mangodt Christophe, Mestdagh Pieter, Michlíková Soña, Oliveira Maria José, Pampaloni Francesco, Piccinini Filippo, Pinheiro Cláudio, Rahn Jennifer, Robbins Stephen M., Siljamaki Elina, Steigemann Patrick, Sys Gwen, Takayama Shuichi, Tesei Anna, Tulkens Joeri, Van Waeyenberge Michiel, Vandesompele Jo, Wagemans Glenn, Weindorfer Claudia, Yigit Nurten, Zablowsky Nina, Zanoni Michele, Blondeel Phillip, De Wever Olivier
Kustantaja: NATURE PORTFOLIO
Julkaisuvuosi: 2021
Journal: Nature Methods
Tietokannassa oleva lehden nimi: NATURE METHODS
Lehden akronyymi: NAT METHODS
Vuosikerta: 18
Numero: 11
Aloitussivu: 1294
Lopetussivu: 1303
Sivujen määrä: 28
ISSN: 1548-7091
eISSN: 1548-7105
DOI: https://doi.org/10.1038/s41592-021-01291-4
Verkko-osoite: https://www.nature.com/articles/s41592-021-01291-4
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/68068844
A knowledgebase developed for increased the transparency of reporting in spheroid research.Spheroids are three-dimensional cellular models with widespread basic and translational application across academia and industry. However, methodological transparency and guidelines for spheroid research have not yet been established. The MISpheroID Consortium developed a crowdsourcing knowledgebase that assembles the experimental parameters of 3,058 published spheroid-related experiments. Interrogation of this knowledgebase identified heterogeneity in the methodological setup of spheroids. Empirical evaluation and interlaboratory validation of selected variations in spheroid methodology revealed diverse impacts on spheroid metrics. To facilitate interpretation, stimulate transparency and increase awareness, the Consortium defines the MISpheroID string, a minimum set of experimental parameters required to report spheroid research. Thus, MISpheroID combines a valuable resource and a tool for three-dimensional cellular models to mine experimental parameters and to improve reproducibility.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
