Vertaisarvioitu alkuperäisartikkeli tai data-artikkeli tieteellisessä aikakauslehdessä (A1)

Organotypic in vitro block culture model to investigate tissue-implant interface. An experimental study on pig mandible




Julkaisun tekijätAreid Nagat, Willberg Jaana, Kangasniemi Ilkka, Närhi Timo O

KustantajaSPRINGER

Julkaisuvuosi2021

JournalJournal of Materials Science: Materials in Medicine

Tietokannassa oleva lehden nimiJOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE

Lehden akronyymiJ MATER SCI-MATER M

Artikkelin numeroARTN 136

Volyymi32

Julkaisunumero11

Sivujen määrä10

ISSN0957-4530

DOIhttp://dx.doi.org/10.1007/s10856-021-06608-5

Verkko-osoitehttps://doi.org/10.1007/s10856-021-06608-5

Rinnakkaistallenteen osoitehttps://research.utu.fi/converis/portal/detail/Publication/67856405


Tiivistelmä
In vitro studies of implant-tissue attachment are primarily based on two-dimensional cell culture models, which fail to replicate the three-dimensional native human oral mucosal tissue completely. Thus, the present study aimed to describe a novel tissue culture model using pig mandibular block including alveolar bone and gingival soft tissues to evaluate the tissue attachment to titanium implant provided with hydrothermally induced TiO2 coating. Tissue attachment on TiO2 coated and non-coated implants were compared. Ti-6Al-4V alloy posts were used to function as implants that were inserted in five pig mandibles. Implants were delivered with two different surface treatments, non-coated (NC) titanium and hydrothermal induced TiO2 coated surfaces (HT). The tissue-implant specimens were cultured at an air/liquid interface for 7 and 14 days. The tissue-implant interface was analyzed by histological and immunohistochemical stainings. The microscopic evaluation suggests that pig tissue explants established soft and hard tissue attachment to both implant surfaces. The epithelial cells appeared to attach to the coated implant. The epithelium adjacent to the implant abutment starts to change its phenotype during the early days of the healing process. New bone formation was seen within small pieces of bone in close contact with the coated implant. In conclusion, this in vitro model maintains the viability of pig tissue and allows histologically and immunohistochemically evaluate the tissue-implant interface. HT-induced TiO2 coating seems to have a favorable tissue response. Moreover, this organotypic tissue culture model is applicable for further studies with quantitative parameters to evaluate adhesion molecules present at the implant-tissue interface.[GRAPHICS].

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Last updated on 2022-07-04 at 16:21