A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä

Inhibition of cathepsin-K and matrix metalloproteinase by photodynamic therapy




TekijätKara Ozlem, Seseogullari Dirihan Roda, Sayin Ozel Gulsum, Tezvergil Mutluay Arzu, Usumez Aslihan

KustantajaElsevier Inc.

Julkaisuvuosi2021

JournalDental Materials

Tietokannassa oleva lehden nimiDental Materials

Vuosikerta37

Numero10

Aloitussivue485

Lopetussivue492

eISSN1879-0097

DOIhttps://doi.org/10.1016/j.dental.2021.08.015


Tiivistelmä

Objectives

The objective of this study was to determine the effects of antimicrobial photodynamic therapy (aPDT) with indocyanine green (ICG) and toluidine blue (TB) on protease activity (matrix-bound cathepsin K and matrix metalloproteinase (MMP) and dentin bond strength.

Methods

Caries-free human third molars were assigned to five groups: 1—control group, 2—application of ICG with activation using an 810 nm diode (aPDT), 3—application of ICG, 4—application of TB with activation using a 660 nm diode (aPDT), and 5—application of TB. For the enzymatic investigation, dentin beams were incubated for either 3 days or 3 weeks. Aliquots of the incubation media were analyzed by ELISA for CTX (C-terminal cross-linked telopeptide of type I Collagen) and ICTP (cross-linked carboxy-terminal telopeptide of type I collagen). For microtensile bond strength testing (μTBS), composite resins were layered onto the tooth surface; the samples were then subjected to μTBS. Kruskall–Wallis and Mann–Whitney U tests were applied for statistical analysis of CTX and ICTP, one way-ANOVA and Tukey’s test were applied for statistical analysis of μTBS.

Results

Pretreating the dentin matrices with aPDT decreased the endogenous protease activity. ICG with laser activation resulted in the highest μTBS. Therefore, aPDT should be considered as a treatment method because it can reduce MMP-mediated dentin degradation and increase the μTBS.

Significance

Inhibiting endogenous protease activity improves the stability of the dentin–adhesive bond and the durability of the bond strength.



Last updated on 2024-26-11 at 13:14