Vertaisarvioitu alkuperäisartikkeli tai data-artikkeli tieteellisessä aikakauslehdessä (A1)
Perfluoroalkyl substances are increased in patients with late-onset ulcerative colitis and induce intestinal barrier defects ex vivo in murine intestinal tissue
Julkaisun tekijät: Fart Frida, Salihović Samira, McGlinchey Aidan, Gareau Melanie G, Orešič Matej, Halfvarson Jonas, Hyötyläinen Tuulia, Schoultz Ida
Kustantaja: TAYLOR & FRANCIS LTD
Julkaisuvuosi: 2021
Journal: Scandinavian Journal of Gastroenterology
Tietokannassa oleva lehden nimi: SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
Lehden akronyymi: SCAND J GASTROENTERO
Sivujen määrä: 10
ISSN: 0036-5521
eISSN: 1502-7708
DOI: http://dx.doi.org/10.1080/00365521.2021.1961306
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/66910971
Background
Environmental factors are strongly implicated in late-onset of inflammatory bowel disease. Here, we investigate whether high levels of perfluoroalkyl substances are associated with (1) late-onset inflammatory bowel disease, and (2) disturbances of the bile acid pool. We further explore the effect of the specific perfluoroalkyl substance perfluorooctanoic acid on intestinal barrier function in murine tissue.
Methods
Serum levels of perfluoroalkyl substances and bile acids were assessed by ultra-performance liquid chromatography coupled to a triple-quadrupole mass spectrometer in matched samples from patients with ulcerative colitis (n = 20) and Crohn's disease (n = 20) diagnosed at the age of >= 55 years. Age and sex-matched blood donors (n = 20), were used as healthy controls. Ex vivo Ussing chamber experiments were performed to assess the effect of perfluorooctanoic acid on ileal and colonic murine tissue (n = 9).
Results
The total amount of perfluoroalkyl substances was significantly increased in patients with ulcerative colitis compared to healthy controls and patients with Crohn's disease (p < .05). Ex vivo exposure to perfluorooctanoic acid induced a significantly altered ileal and colonic barrier function. The distribution of bile acids, as well as the correlation pattern between (1) perfluoroalkyl substances and (2) bile acids, differed between patient and control groups.
Discussion
Our results demonstrate that perfluoroalkyl substances levels are increased in patients with late-onset ulcerative colitis and may contribute to the disease by inducing a dysfunctional intestinal barrier.
Ladattava julkaisu This is an electronic reprint of the original article. |