A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Structural variability, coordination and adaptation of a native photosynthetic machinery
Tekijät: Zhao LS, Huokko T, Wilson S, Simpson DM, Wang Q, Ruban AV, Mullineaux CW, Zhang YZ, Liu LN
Kustantaja: NATURE RESEARCH
Julkaisuvuosi: 2020
Journal: Nature Plants
Tietokannassa oleva lehden nimi: NATURE PLANTS
Lehden akronyymi: NAT PLANTS
Vuosikerta: 6
Numero: 7
Aloitussivu: 869
Lopetussivu: 882
Sivujen määrä: 17
ISSN: 2055-026X
DOI: https://doi.org/10.1038/s41477-020-0694-3
Tiivistelmä
High-resolution atomic force microscopy is used to visualize photosynthetic supercomplexes within the thylakoid membranes of a cyanobacterium. The organizational variability of these supercomplexes permits efficient linear and cyclic electron transport as well as bioenergetic regulation.Cyanobacterial thylakoid membranes represent the active sites for both photosynthetic and respiratory electron transport. We used high-resolution atomic force microscopy to visualize the native organization and interactions of photosynthetic complexes within the thylakoid membranes from the model cyanobacteriumSynechococcus elongatusPCC 7942. The thylakoid membranes are heterogeneous and assemble photosynthetic complexes into functional domains to enhance their coordination and regulation. Under high light, the chlorophyll-binding proteins IsiA are strongly expressed and associate with Photosystem I (PSI), forming highly variable IsiA-PSI supercomplexes to increase the absorption cross-section of PSI. There are also tight interactions of PSI with Photosystem II (PSII), cytochromeb(6)f, ATP synthase and NAD(P)H dehydrogenase complexes. The organizational variability of these photosynthetic supercomplexes permits efficient linear and cyclic electron transport as well as bioenergetic regulation. Understanding the organizational landscape and environmental adaptation of cyanobacterial thylakoid membranes may help inform strategies for engineering efficient photosynthetic systems and photo-biofactories.
High-resolution atomic force microscopy is used to visualize photosynthetic supercomplexes within the thylakoid membranes of a cyanobacterium. The organizational variability of these supercomplexes permits efficient linear and cyclic electron transport as well as bioenergetic regulation.Cyanobacterial thylakoid membranes represent the active sites for both photosynthetic and respiratory electron transport. We used high-resolution atomic force microscopy to visualize the native organization and interactions of photosynthetic complexes within the thylakoid membranes from the model cyanobacteriumSynechococcus elongatusPCC 7942. The thylakoid membranes are heterogeneous and assemble photosynthetic complexes into functional domains to enhance their coordination and regulation. Under high light, the chlorophyll-binding proteins IsiA are strongly expressed and associate with Photosystem I (PSI), forming highly variable IsiA-PSI supercomplexes to increase the absorption cross-section of PSI. There are also tight interactions of PSI with Photosystem II (PSII), cytochromeb(6)f, ATP synthase and NAD(P)H dehydrogenase complexes. The organizational variability of these photosynthetic supercomplexes permits efficient linear and cyclic electron transport as well as bioenergetic regulation. Understanding the organizational landscape and environmental adaptation of cyanobacterial thylakoid membranes may help inform strategies for engineering efficient photosynthetic systems and photo-biofactories.
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