A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

Cancer cell line microarray as a novel screening method for identification of radioresistance biomarkers in head and neck squamous cell carcinoma




Julkaisun tekijät: Routila Johannes, Suvila Karri, Grénman Reidar, Leivo Ilmo, Westermarck Jukka, Ventelä Sami

Kustantaja: biomed central

Julkaisuvuosi: 2021

Journal: BMC Cancer

Lehden akronyymi: BMC Cancer

Volyymi: 21

eISSN: 1471-2407

DOI: http://dx.doi.org/10.1186/s12885-021-08618-6


Tiivistelmä

Background: Currently, no clinically useful biomarkers for radioresistance are available in head and neck squamous cell carcinoma (HNSCC). This study assesses the usefulness of Cell Line Microarray (CMA) method to enhance immunohistochemical screening of potential immunohistochemical biomarkers for radioresistance in HNSCC cell lines.

Methods: Twenty-nine HNSCC cell lines were cultured, cell pellets formalin-fixed, paraffin-embedded, and arrayed. Radioresistance features of the cell lines were combined to immunohistochemical stains for p53, NDFIP1, EGFR, stem cell marker Oct4, and PP2A inhibitor CIP2A.

Results: Expression of p53, EGFR or CIP2A did not indicate intrinsic radioresistance in vitro. Stem cell marker Oct4 nuclear positivity and NDFIP1 nuclear positivity was correlated with increased intrinsic radioresistance.

Conclusion: The usefulness of CMA in analysis of HNSCC cell lines and discovery of biomarkers is demonstrated. CMA is very well adapted to both testing of antibodies in a large panel of cell lines as well as correlating staining results with other cell line characteristics. In addition, CMA-based antibody screening proved an efficient and relatively simple method to identify potential radioresistance biomarkers in HNSCC.


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Last updated on 2021-05-08 at 13:59