Methods to Monitor Ras Activation State

: Kopra Kari, Härmä Harri

: Ignacio Rubio, Ian Prior

Publisher: Humana Press Inc.

: 2021

: Ras Activity and Signaling: Methods and Protocols

: Methods in Molecular Biology

: 2262

: 137

: 167

: 978-1-0716-1189-0

: 978-1-0716-1190-6

: 1064-3745

DOI: https://doi.org/10.1007/978-1-0716-1190-6_8

Environment-sensitive labels, Fluorescence polarization (FP), Förster resonance energy transfer (FRET), GTP- and GDP-specific antibodies, Quenching resonance energy transfer (QRET), Ras-binding domain (RBD), Small GTPases, Thermal stability assay (TSA), Time-resolved fluorescence resonance energy transfer (TR-FRET)