A1 Journal article – refereed

ACONITASE 3 is part of the ANAC017 transcription factor-dependent mitochondrial dysfunction response

List of Authors: Pascual Jesús, Rahikainen Moona, Angeleri Martina, Alegre Sara, Gossens Richard, Shapiguzov Alexey, Heinonen Arttu, Trotta Andrea, Durian Guido, Winter Zsófia, Sinkkonen Jari, Kangasjärvi Jaakko, Whelan James, Kangasjärvi Saijaliisa

Publication year: 2021

Journal: Plant Physiology

eISSN: 1532-2548

DOI: http://dx.doi.org/10.1093/plphys/kiab225


Mitochondria are tightly embedded within metabolic and regulatory networks that optimize plant performance in response to environmental challenges. The best-known mitochondrial retrograde signaling pathway involves stress-induced activation of the transcription factor NAC DOMAIN CONTAINING PROTEIN 17 (ANAC017), which initiates protective responses to stress-induced mitochondrial dysfunction in Arabidopsis (Arabidopsis thaliana). Post-translational control of the elicited responses, however, remains poorly understood. Previous studies linked protein phosphatase 2A subunit PP2A-B’γ, a key negative regulator of stress responses, with reversible phosphorylation of ACONITASE 3 (ACO3). Here we report on ACO3 and its phosphorylation at Ser91 as key components of stress regulation that are induced by mitochondrial dysfunction. Targeted mass spectrometry-based proteomics revealed that the abundance and phosphorylation of ACO3 increased under stress, which required signaling through ANAC017. Phosphomimetic mutation at ACO3-Ser91 and accumulation of ACO3S91D-YFP promoted the expression of genes related to mitochondrial dysfunction. Furthermore, ACO3 contributed to plant tolerance against UV-B or antimycin A-induced mitochondrial dysfunction. These findings demonstrate that ACO3 is both a target and mediator of mitochondrial dysfunction signaling, and critical for achieving stress tolerance in Arabidopsis leaves.

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Last updated on 2021-15-09 at 09:57