A1 Refereed original research article in a scientific journal
Determination of serum neutralizing antibodies reveals important difference in quality of antibodies against pertussis toxin in children after infection
Authors: Zhang Yuxiao, Li Yarong, Chen Zhiyun, Liu Xiaoguai, Peng Xiaokang, He Qiushui
Publisher: ELSEVIER SCI LTD
Publication year: 2021
Journal: Vaccine
Journal name in source: VACCINE
Journal acronym: VACCINE
Volume: 39
Issue: 13
First page : 1826
Last page: 1830
Number of pages: 5
ISSN: 0264-410X
eISSN: 1873-2518
DOI: https://doi.org/10.1016/j.vaccine.2021.02.045
Objectives: To determine neutralizing antibodies to pertussis toxin (PTNAs) in children with suspected pertussis and to compare results of PTNAs and anti-PT IgG antibodies.
Methods: 172 hospitalized children with suspected pertussis were included. Pertussis was confirmed by culture, PCR and/or serology. PTNAs were determined by Chinese hamster ovary (CHO) cell assay.
Results: A correlation between titers of PTNAs and anti-PT IgG levels was noticed in 172 patients (Spearman R = 0.68, P < 0.001). Subjects with same concentrations of anti-PT IgG antibodies could have different titers of PTNAs and the maximum difference observed reached to 1024 times in ELISA-confirmed patients. Moreover, subjects with same titers of PTNAs could have different concentrations of anti-PT IgG antibodies.
Conclusions: Our results indicated that in some children high concentrations of anti-PT IgG antibodies do not always mean effective PTNAs induced after infection, stressing the importance of detecting PTNAs after infection and vaccination. Clinical trial registry: Not applicable.
